Article 2026 · 0 citations

Chicken feather protein hydrolysate: An alternative for the prevention of indomethacin-induced gastric ulcer formation in rats

Doymus, Meryem, Dasdemir, Elanur, Sengul, Emin, Yildirim, Serkan, Tekin, Samet, Arslan, Nazli Pinar, Bolat, Ismail, Kiliclioglu, Metin, Ozkan, Hakan, Taskin, Mesut

Abstract

This study examined the anti-ulcer potential of chicken feather protein hydrolysate (Hyd) against indomethacin (Ind)-induced gastric ulcer. Hyd was prepared from feathers by microbial fermentation technique using locally isolated Bacillus licheniformis EYT2 (GenBank accession number: PV612017). Hyd was orally administrated to the rats at the doses of 100 mg/kg BW (low-dose group, Hyd100) and 200 mg/kg BW (high-dose group, Hyd200) before Ind treatment. The effectiveness of Hyd was compared with Ranitidin (Ran). Six experimental groups were designed: Control, Ind, Ran+Ind, Hyd100 + Ind, Hyd200 + Ind, and Ran+Hyd200 + Ind. Hyd was determined to have a high protein content (81.6%) and exhibit high water solubility in a wide pH range from 1 to 10. Ind-treatment caused severe erosion, ulceration, and degeneration in gastric tissue, elevated the levels of malondialdehyde, pro-inflammatory cytokines, inducible nitric oxide synthase, 8 hydroxy-2 '-deoxyguanosine, Bax, caspase-3, nuclear factor kappa B (NF-kB) and mitogen-activated protein kinase-38 (MAPK-38) and reduced the levels of glutathione, antioxidant enzymes, anti-inflammatory cytokine IL-10, prostaglandin E2, anti-apoptotic gene Bcl-2 and nuclear factor erythroid 2-related factor 2 (Nrf2). Conversely, Hyd administration, especially high dose Hyd (Hyd200 + Ind group) reversed these alterations. Furthermore, a combination of Ran and high-dose Hyd (Ran+Hyd200 + Ind) completely prevented Ind toxicity. The protective effect of Hyd was attributed to its antioxidant, antiapoptotic and anti-inflammatory activites as well as prostaglandin synthesis-enhancing property. These results imply that Hyd may be used as a supplement with anti-ulcer activity in alternative medicine and/or as a protein source in animal and human nutrition. This is the first report on anti-ulcer potential of Hyd.

Keywords

Bacillus licheniformis Protein hydrolysate Antioxidant activity Anti-inflammatory activity Anti-ulcer OXIDATIVE STRESS NITRIC-OXIDE ANTIOXIDANT PEPTIDES PEPTONE WASTE ACID FERMENTATION DEGRADATION NITROGEN

WoS Categories

Food Science & Technology

Citation

Journal: FOOD RESEARCH INTERNATIONAL
Year: 2026
Volume: 231
ISSN: 0963-9969
eISSN: 1873-7145
DOI: 10.1016/j.foodres.2026.118789
WoS UID: WOS:001703637500001

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