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Makale Tam Makale Uluslararası 2005

The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo

Olcay HISAR, S BEYDEMIR, İlhami Gülçin, SA HISAR, Telat Yanık, Ömer İrfan Küfrevioğlu

Turkish Journal Of Veterinary Sciences Cilt 29 · Sayı 3 · ss. 841–845 · 01.01.2005

Özet

The effects of melatonin hormone on rainbow trout (Oncorhynchus mykiss) erythrocyte carbonic anhydrase enzyme purified by using Sepharose 4B-L tyrosine-sulfanylamide affinity gel chromatography were investigated in vitro and in vivo. Carbonic anhydrase from Oncorhynchus mykiss erythrocytes was purified with a specific activity of similar to 400 EU/mg protein and similar to 200-fold in a yield of similar to 20. Melatonin did not inhibit carbonic anhydrase enzyme in vitro. Carbonic anhydrase activity of the control group was determined as 1597.7 +/- 429.0 EU/g hemoglobin in vivo. Carbonic anhydrase activities were measured at 1, 3 and 5 h by following melatonin injection. The corresponding mean activities of melatonin were 1406.4 +/- 372.1, 1346.3 +/- 477.4 and 783.8 +/- 494.3 EU/g hemoglobin respectively. Oncorhynchus mykiss erythrocyte carbonic anhydrase was inhibited significantly by melatonin hormone 5 h after injection in vivo (P < 0.05). Data from the present study suggest that excessive usage of this hormone should be avoided in fish reproduction.

Anahtar kelimeler

IN-VITRO Maturation melatonin carbonic anhydrase INHIBITION ERYTHROCYTE IN-VIVO FISH Oncorhynchus mykiss PINEAL CO2

Atıf için künye

HISAR, O., BEYDEMIR, S., Gülçin, İ., HISAR, S., Yanık, T., & Küfrevioğlu, Ö. İ. (2005). The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo. Turkish Journal Of Veterinary Sciences, 29(3), 841–845.

BibTeX 
@article{hisar2005-67712,
  author = {HISAR, Olcay and BEYDEMIR, S and Gülçin, İlhami and HISAR, SA and Yanık, Telat and Küfrevioğlu, Ömer İrfan},
  title = {The effects of melatonin hormone on carbonic anhydrase enzyme activity in rainbow trout (Oncorhynchus mykiss) erythrocytes in vitro and in vivo},
  journal = {Turkish Journal Of Veterinary Sciences},
  year = {2005},
  volume = {29},
  number = {3},
  pages = {841--845},
  issn = {1300-0128},
  url = {https://akademik.atauni.edu.tr/yayinlar/67712-the-effects-of-melatonin-hormone-on-carbonic-anhydrase-enzyme-activity-in}
}