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Makale Tam Makale Uluslararası 2025

Liquid Chromatography-Tandem Mass Spectrometric (LC-MS/MS) Determination of Allergenic Proteins in Edible Flour Derived from Yellow Mealworms

Konstantina Papastavropoulou, Stella Mavrakaki, Marios Kostakis, Anastasia Koupa, Nikolaos S. Thomaidis, Antony C. Calokerinos, Emel Öz, Fatih Öz, Charalampos Proestos

ANALYTICAL LETTERS Cilt 58 · Sayı 18 · ss. 3176–3192 · 01.01.2025
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Özet

Entomophagy offers significant benefits but also poses allergen risks. This study examined the presence of allergenic proteins in freeze-dried yellow mealworm powder using LC-MS/MS with electrospray ionization in positive mode. Allergenic peptides were identified and compared with plant- and animal-based allergens listed under EU Regulation 1169/2011, utilizing certified reference materials. The findings reveal the presence of tropomyosin, which may trigger allergic reactions in individuals with crustacean allergies. The larvae’s diet did not significantly influence allergen content. This research underscores the need for robust legislation to ensure safety in insect production and consumption, supporting the growth of the edible insect industry.

Anahtar kelimeler

High–performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) insect allergenic proteins tropomyosin yellow mealworm allergens

Atıf için künye

Papastavropoulou, K., Mavrakaki, S., Kostakis, M., Koupa, A., Thomaidis, N. S., Calokerinos, A. C., Öz, E., Öz, F., & Proestos, C. (2025). Liquid Chromatography-Tandem Mass Spectrometric (LC-MS/MS) Determination of Allergenic Proteins in Edible Flour Derived from Yellow Mealworms. ANALYTICAL LETTERS, 58(18), 3176–3192. https://doi.org/10.1080/00032719.2025.2456683

BibTeX
@article{papastavropoulou2025-262820,
  author = {Papastavropoulou, Konstantina and Mavrakaki, Stella and Kostakis, Marios and Koupa, Anastasia and Thomaidis, Nikolaos S. and Calokerinos, Antony C. and Öz, Emel and Öz, Fatih and Proestos, Charalampos},
  title = {Liquid Chromatography-Tandem Mass Spectrometric (LC-MS/MS) Determination of Allergenic Proteins in Edible Flour Derived from Yellow Mealworms},
  journal = {ANALYTICAL LETTERS},
  year = {2025},
  volume = {58},
  number = {18},
  pages = {3176--3192},
  issn = {0003-2719},
  doi = {10.1080/00032719.2025.2456683},
  url = {https://akademik.atauni.edu.tr/yayinlar/262820-liquid-chromatography-tandem-mass-spectrometric-lc-msms-determination-of}
}