Prevalence of Colistin Resistance in Escherichia coli in Eastern Turkey and Genomic Characterization of an mcr-1 Positive Strain from Retail Chicken Meat

Adiguzel, Mehmet Cemal; Baran, Alper; Wu, Z; Cengiz, Seyda; Dai, L; Öz, Cihan; Ozmenli, E; Goulart, DB; Sahin, O

doi:10.1089/mdr.2020.0209

Makale Tam Makale Uluslararası 2021

Prevalence of Colistin Resistance in Escherichia coli in Eastern Turkey and Genomic Characterization of an mcr-1 Positive Strain from Retail Chicken Meat

Mehmet Cemal Adiguzel, Alper Baran, Z Wu, Seyda Cengiz, L Dai, Cihan Öz, E Ozmenli, DB Goulart, O Sahin

Microbial Drug Resistance Cilt 27 · Sayı 3 · ss. 424–432 · 01.03.2021

DOI ile aç

Özet

© Copyright 2021, Mary Ann Liebert, Inc., publishers 2021.Colistin is one of the most effective antibiotics against multidrug resistant Gram-negative bacteria. However, the recent emergence of plasmid-borne mobilized colistin resistance (mcr) genes is considered a serious antimicrobial resistance challenge worldwide. In this study, we report detection of an mcr-1 carrying Escherichia coli isolate (named ATAVET mcr-1 Turkey) from retail raw chicken meat in Turkey. Of the 11 (from 500 total tested) phenotypically colistin-resistant isolates, 1 was shown to carry the mcr-1 gene by PCR. Whole-genome sequencing indicated that mcr-1 was located on a ∼13 kb-long contig that was almost identical to the corresponding part in pZJ1635, an IncI2 plasmid encoding mcr-1 in the same genetic context in another E. coli strain. In addition, ATAVET mcr-1 Turkey harbored blaCTX-M-8, qnrB19, mdf(A), tet(A), sul2, aph(3″)-Ib, aph(6)-Id, and floR resistance genes. Phylogenetic analysis based on whole genome and multilocus sequence typing indicated that ATAVET mcr-1 Turkey was more closely related to mcr-1 carrying E. coli isolates from food and human clinical samples previously reported from different parts of the world than to those from Turkey. These findings further emphasize the worldwide emergence and spread of mcr meditated colistin resistance in bacteria with zoonotic potential within animals and the food chain.

Anahtar kelimeler

ESCHERICHIA-COLI Colistin GENOMIC CHARACTERIZATION mcr-1gene phylogenetic analysis

Atıf için künye

Adiguzel, M. C., Baran, A., Wu, Z., Cengiz, S., Dai, L., Öz, C., Ozmenli, E., Goulart, D., & Sahin, O. (2021). Prevalence of Colistin Resistance in Escherichia coli in Eastern Turkey and Genomic Characterization of an mcr-1 Positive Strain from Retail Chicken Meat. Microbial Drug Resistance, 27(3), 424–432. https://doi.org/10.1089/mdr.2020.0209

BibTeX

@article{adiguzel2021-250749,
  author = {Adiguzel, Mehmet Cemal and Baran, Alper and Wu, Z and Cengiz, Seyda and Dai, L and Öz, Cihan and Ozmenli, E and Goulart, DB and Sahin, O},
  title = {Prevalence of Colistin Resistance in Escherichia coli in Eastern Turkey and Genomic Characterization of an mcr-1 Positive Strain from Retail Chicken Meat},
  journal = {Microbial Drug Resistance},
  year = {2021},
  volume = {27},
  number = {3},
  pages = {424--432},
  issn = {1076-6294},
  doi = {10.1089/mdr.2020.0209},
  url = {https://akademik.atauni.edu.tr/yayinlar/250749-prevalence-of-colistin-resistance-in-escherichia-coli-in-eastern-turkey-and}
}