Global warming and glyphosate toxicity (II): Offspring zebrafish modelling with behavioral, morphological and immunohistochemical approaches

The increase in temperature due to global warming greatly affects the toxicity produced by pesticides in the aquatic ecosystem. Studies investigating the effects of such environmental stress factors on next generations are important in terms of the sustainability of ecosystems. In this study, the effects of parental synergistic exposure to glyphosate and temperature increase on the next generation were investigated in a zebrafish model. For this purpose, adult zebrafish were exposed to 1 ppm and 5 ppm glyphosate for 96 h at four different temperatures (28.5, 29.0, 29.5, 30.0 degrees C). At the end of this period, some of the fish were subjected to the recovery process for 10 days. At the end of both treatments, a new generation was taken from the fish and morphological, physiological, molecular and behav-ioral analysis were performed on the offspring. According to the results, in parallel with the 0.5-degree temperature increase applied to the parents with glyphosate exposure, lower survival rate, delay in hatching, increased body mal-formations and lower blood flow and heart rate were detected in the offspring. In addition, according to the results of whole mouth larva staining, increased apoptosis, free oxygen radical formation and lipid accumulation were detected in the offspring. Moreover, it has been observed that the temperature increases to which the parents are exposed affects the light signal transmission and serotonin pathways in the offspring, resulting in more dark/light locomotor activity and increased thigmotaxis.