Production of cost-effective rhamnolipid from <i>Halopseudomonas sabulinigri</i> OZK5 using waste frying oil
Baltaci, Mustafa Ozkan, Albayrak, Seyda, Akbulut, Sumeyye, Dasdemir, Elanur, Ozkan, Hakan, Adiguzel, Ahmet, Taskin, Mesut
The major barrier to the wide-range application of biosurfactants is their high cost of production and low yield. In this study, waste frying oil (WFO) was used as the sole carbon source to produce cost-effective and eco-friendly rhamnolipids by Halopseudomonas sabulinigri OZK5 isolated from crude oil-contaminated soil samples. The optimal culture conditions for rhamnolipid production were determined as 30 ml/l waste frying oil, 37 degrees C temperature, pH 8, and 72 h incubation time. Under the optimized conditions 2.97 g/l rhamnolipid production was achieved. With a critical micelle concentration of 50 mg/l, the rhamnolipids could reduce the surface tension of water to 37.5 mN/m and demonstrate strong emulsifying activity (E24 = 67.3%). As a result of FTIR analyses, major peaks were obtained at 2924, 2854, 1720, 1570, 1396, 1051, and 981 cm-1. In conclusion, rhamnolipid production by non-pathogenic Halopseudomonas sabulinigri OZK5 using a low-cost fermentation medium has been shown to be biotechnologically promising.
- Journal
- INTERNATIONAL MICROBIOLOGY
- Year
- 2025
- Volume
- 28
- Issue
- 6
- Pages
- 1409-1417
- ISSN
- 1139-6709
- eISSN
- 1618-1905
- DOI
- 10.1007/s10123-024-00630-7
- WoS UID
- WOS:001385949100001