Article Early Access 2026 · 0 atıf

Valorization of fruit pits from Prunus laurocerasus and Prunus cerasifera as agro-food byproducts: Bioactive properties and safety assessment for potential food additive applications

Güven, Zühal Bayrakçeken, Karadayı, Mehmet, Yilmaz, Mustafa Abdullah, Tugay, Osman, Cakir, Oguz

Özet

BACKGROUND Food additives are widely used to extend the shelf life of foods and maintain their quality. In this study, the potential of Prunus laurocerasus and Prunus cerasifera fruit pits (endocarp and seed) as food additives was investigated in terms of cytotoxicity, antigenotoxicity, antioxidant activity, enzymatic anti-browning, and urease enzyme inhibition, and their phytochemical contents were comprehensively analyzed.RESULTS While the extracts showed low cytotoxicity against the healthy L929 cell line, they exhibited a concentration-dependent increase in selective cytotoxicity against A549, CaCo-2, Hep3B, MCF-7, and PC-3 cells. Furthermore, the genotoxic safety of the extracts was assessed in both prokaryotic and eukaryotic model test systems. The extracts showed no cytogenotoxic or mutagenic effects in the Allium cepa and Ames/Salmonella assays. P. cerasifera endocarp ethyl acetate extract exhibited the highest antioxidant activity (DPPH, CUPRAC, and TEAC: 576.24, 402.42, and 680.14 mg Trolox g-1 extract, respectively) and strong enzyme inhibition, with tyrosinase (IC50: 10.46 +/- 1.48 mu g mL-1) nearly comparable to kojic acid and potent urease inhibition (IC50: 28.60 +/- 2.08 mu g mL-1). Phytochemical analysis of the extracts was performed using liquid chromatography-tandem mass spectrometry, with epicatechin (10.722 mg g-1 extract) detected in the highest amount in the endocarp of P. laurocerasus and quinic acid (11.679 mg g-1 extract) detected in the endocarp of P. cerasifera.CONCLUSION Overall, according to the conducted cytotoxicity and genotoxicity assays, P. laurocerasus and P. cerasifera fruit pits were found to be safe, rich in bioactive phytochemicals, and exhibited strong antioxidant and enzyme inhibitory activities, highlighting their potential as sustainable functional ingredients or natural food additives.

Anahtar Kelimeler

antioxidant antiproliferative food additives Prunus cerasifera Prunus laurocerasus tyrosinase BUTYLATED HYDROXYANISOLE ANTIOXIDANT ACTIVITY LIVESTOCK DISEASES CELL PROLIFERATION OIL

WoS Kategorileri

Agriculture, Multidisciplinary Chemistry, Applied Food Science & Technology

Künye

Dergi: JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
Yıl: 2026
ISSN: 0022-5142
eISSN: 1097-0010
DOI: 10.1002/jsfa.70653
WoS UID: WOS:001737560200001

Yayını aç (DOI)