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Makale Tam Makale Uluslararası 2022

Electrically controlled mRNA delivery using a polypyrrole-graphene oxide hybrid film to promote osteogenic differentiation of human mesenchymal stem cells

Huijung Kim, Kübra Solak, Yoojoong Han, Yeon-Woo Cho, Kyeong-Mo Koo, Chang-Dae Kim, Zhengtang Luo, Hyungbin Son, Hyung-Ryong Kim, Ahmet Mavi, Tae-Hyung Kim

NANO RESEARCH Cilt 15 · Sayı 10 · ss. 9253–9263 · 01.10.2022
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Özet

Direct messenger ribonucleic acid (mRNA) delivery to target cells or tissues has revolutionized the field of biotechnology. However, the applicability of regenerative medicine is limited by the technical difficulties of various mRNA-loaded nanocarriers. Herein, we report a new conductive hybrid film that could guide osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs) via electrically controlled mRNA delivery. To find optimal electrical conductivity and mRNA-loading capacity, the polypyrrole-graphene oxide (PPy-GO) hybrid film was electropolymerized on indium tin oxide substrates. We found that the fluorescein sodium salt, a molecule partially mimicking the physical and chemical properties of mRNAs, can be effectively absorbed and released by electrical stimulation (ES). The hADMSCs cultivated on the PPy-GO hybrid film loaded with pre-osteogenic mRNAs showed the highest osteogenic differentiation under electrical stimulation. This platform can load various types of RNAs thus highly promising as a new nucleic acid delivery tool for the development of stem cell-based therapeutics.

Anahtar kelimeler

mesenchymal stem cells GRAPHENE OXIDE polypyrrole messenger ribonucleic acid (mRNA) delivery electrical release

Sürdürülebilir Kalkınma Amaçları

  • SKA 3: Sağlıklı Bireyler

Atıf için künye

Kim, H., Solak, K., Han, Y., Cho, Y., Koo, K., Kim, C., Luo, Z., Son, H., Kim, H., Mavi, A., & Kim, T. (2022). Electrically controlled mRNA delivery using a polypyrrole-graphene oxide hybrid film to promote osteogenic differentiation of human mesenchymal stem cells. NANO RESEARCH, 15(10), 9253–9263. https://doi.org/10.1007/s12274-022-4613-y

BibTeX 
@article{kim2022-80787,
  author = {Kim, Huijung and Solak, Kübra and Han, Yoojoong and Cho, Yeon-Woo and Koo, Kyeong-Mo and Kim, Chang-Dae and Luo, Zhengtang and Son, Hyungbin and Kim, Hyung-Ryong and Mavi, Ahmet and Kim, Tae-Hyung},
  title = {Electrically controlled mRNA delivery using a polypyrrole-graphene oxide hybrid film to promote osteogenic differentiation of human mesenchymal stem cells},
  journal = {NANO RESEARCH},
  year = {2022},
  volume = {15},
  number = {10},
  pages = {9253--9263},
  issn = {1998-0124},
  doi = {10.1007/s12274-022-4613-y},
  url = {https://akademik.atauni.edu.tr/yayinlar/80787-electrically-controlled-mrna-delivery-using-a-polypyrrole-graphene-oxide-hybrid}
}