Enzyme Inhibition PropertyInvestigation of different daisies; Calendula officinalis, Matricaria chamomilla, and Anthemis pseudocotula from Asteraceae family

The Asteraceae family is one of the largest flowering plant families, and most of these family members

have therapeutic applications and a long history in traditional medicine. Here, enzyme inhibition

properties of three different genera from the Asteraceae family, namely, Calendula officinalis,

Matricaria chamomilla, and Anthemis pseudocotula were investigated for potential use in medicine.

Enzyme inhibition studies over human carbonic anhydrase I and II, acetylcholine esterase, α-amylase,

and α-glycosidase were performed, and IC50 values were calculated. Then, the phytochemical contents

of each plant were determined through LC-MS/MS against 33 well-known plant secondary

metabolites, and the major compounds were subjected to molecular docking studies to estimate

potential inhibition mechanisms. AutoDock Vina 1.1.2. The best docking scores and binding

interactions over acetylcholinesterase (AChE), α-glycosidase (AG), carbonic anhydrase I (hCA I) and

II (hCA II), and α-amylase (AA) are analyzed by BIOVIA Discovery Studio.