MODELLING OF PHENAZINE-1-CARBOXYLIC ACID PRODUCTION BY PSEUDOMONAS CHLORORAPSIS SUBSP. AUREOFACIENS IN SUBMERGED CULTURES AND INVESTIGATION OF ITS ANTIMICROBIAL AND ANTICANCER ACTIVITY

Phenazines and derivatives are nitrogen-containing heterocyclic compounds that are

synthesized by Pseudomonas and Streptomyces species. These molecules are redox

agents with broad-spectrum anti-microbial, anti-tumor and anti-parasitic activity.

Over the past decades, there has been great interest in their biotechnological

production because of their unique properties. Bacterial fermentation processes have

advantages over chemical synthesis, since necessity of the highly toxic substrates

and harsh conditions during the chemical synthesis, formation of toxic by-products

and also low productivity. The aim of the study is to produce phenazine-1 carboxylic

acid (PCA) by bacterial fermentation as a bioactive agent. For this purpose, PCA was

produced by P. chlororapsis subsp. aureofaciens on the batch mode in submerged

cultures. Modelling of PCA production and growth of organisms was determined by

temporal analysis of variations of biomass, product, depleted carbon and nitrogen

source. Chromatographic and spectroscopic methods were used for purification,

quantification and characterization of PCA produced by fermentation. Besides, in

vitro anti-cancer and antimicrobial activity of characterized pigment was also

investigated.