Purification and Characterization of Laccase from newly isolated Thermophilic Brevibacillus sp (Z1) and its applications in removal of Textile Dyes
Özet
Laccases are multicopper-containing oxidases (EC 1.10.3.2.) and they are widely found in fungi, higher plants and bacteria. Because of their high pH stability and thermostability, laccases from this strain have a potential for the treatment of textile bleaching effluents. In this research, the thermophilic test strain isolated from the Agri-Diyadin thermal springs, in the east of Turkey, were subjected to analysis of 16S rRNA sequence. The obtained sequence data were compared with data from the database in the Genebank and as a result it has been identified that this organism is a member of Brevibacillus genus (GenBank number KC292196). The laccase from Brevibacillus sp. (Z1) was purified by using precipitate of saturation (NH4)(2)SO4, DEAE-cellulose and Sephacryl S200. The purification degree and the molecular mass of the enzyme (93 and 110 kDa) were determined by SDS-PAGE and gel filtration chromatography.
Anahtar kelimeler
Atıf için künye
BOZOGLU, C., Adıgüzel, A., Nadaroğlu, H., YANMIS, D., & GÜLLÜCE, M. (2013). Purification and Characterization of Laccase from newly isolated Thermophilic Brevibacillus sp (Z1) and its applications in removal of Textile Dyes. RESEARCH JOURNAL OF BIOTECHNOLOGY, 8(9), 56–66.
BibTeX
@article{bozoglu2013-76150,
author = {BOZOGLU, Ceyda and Adıgüzel, Ahmet and Nadaroğlu, Hayrunnisa and YANMIS, Derya and GÜLLÜCE, Medine},
title = {Purification and Characterization of Laccase from newly isolated Thermophilic Brevibacillus sp (Z1) and its applications in removal of Textile Dyes},
journal = {RESEARCH JOURNAL OF BIOTECHNOLOGY},
year = {2013},
volume = {8},
number = {9},
pages = {56--66},
issn = {2278-4535},
url = {https://akademik.atauni.edu.tr/yayinlar/76150-purification-and-characterization-of-laccase-from-newly-isolated-thermophilic}
}