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Makale Tam Makale Uluslararası 2014

Molecular Typing of Thermophilic Bacilli isolated from Different Hot Springs of Turkey

Derya YANMIS, Ahmet Adıgüzel

RESEARCH JOURNAL OF BIOTECHNOLOGY Cilt 9 · Sayı 10 · ss. 83–88 · 01.10.2014

Özet

In this study, the molecular characterization of 10 thermophilic bacilli isolated from different geothermal areas in Turkey was done by using the fatty acid methyl ester, 16S rRNA sequencing and rep-PCR profiling methods. Depending upon the data obtained from fatty acid analysis, it was determined that there were 8 different fatty acids in the examined 10 bacterial strains and 16:0 and 17:0 anteiso were determined to be the fundamental fatty acid. In consequence of the comparison of 16SrRNA sequence analysis results with the data in GenBank, all the isolates were identified to resemble Aeribacillus pallidus at a rate of >= 98 %. Later on, rep-PCR was performed for the purpose of determining the differences between these isolates.

Anahtar kelimeler

PCR Erzurum DIVERSITY REP-PCR 16S rRNA sequencing FAMEs thermophilic bacteria RETAIL BEEF GEOBACILLUS STRAINS DEGREES-C STEAROTHERMOPHILUS

Atıf için künye

YANMIS, D., & Adıgüzel, A. (2014). Molecular Typing of Thermophilic Bacilli isolated from Different Hot Springs of Turkey. RESEARCH JOURNAL OF BIOTECHNOLOGY, 9(10), 83–88.

BibTeX
@article{yanmis2014-76119,
  author = {YANMIS, Derya and Adıgüzel, Ahmet},
  title = {Molecular Typing of Thermophilic Bacilli isolated from Different Hot Springs of Turkey},
  journal = {RESEARCH JOURNAL OF BIOTECHNOLOGY},
  year = {2014},
  volume = {9},
  number = {10},
  pages = {83--88},
  issn = {0973-6263},
  url = {https://akademik.atauni.edu.tr/yayinlar/76119-molecular-typing-of-thermophilic-bacilli-isolated-from-different-hot-springs-of}
}