Effects of some drugs on human erythrocyte glucose 6-phosphate dehydrogenase: an in vitro study
Özet
Inhibitory effects of some drugs on glucose 6-phosphate dehydrogenase from the erythrocytes of human have been investigated. For this purpose, at the beginning, erythrocyte glucose 6-phosphate dehydrogenase was purified 2256 times in a yield of 44.22% by using ammonium sulphate precipitation and 2',5'-ADP Sepharose 4B affinity gel. Temperature of +4 degrees C was maintained during the purification process. Enzyme activity was determined with the Beutler method by using a spectrophotometer at 340 nm. This method was utilized for all kinetic studies. Ketotifen, dacarbazine, thiocolchicoside, meloxicam, methotrexate, furosemide, olanzapine, methylprednizolone acetate, paricalcitol, ritodrine hydrochloride, and gadobenate-dimeglumine were used as drugs. All the drugs indicated the inhibitory effects on the enzyme. Ki constants for glucose 6-phosphate dehydrogenase were found by means of Lineweaver-Burk graphs. While methylprednizolone acetate showed competitive inhibition, the others displayed non-competitive inhibition.
Anahtar kelimeler
Atıf için künye
Akkemik, E., Budak, H., & ÇİFTÇİ, M. (2010). Effects of some drugs on human erythrocyte glucose 6-phosphate dehydrogenase: an in vitro study. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 25(6), 871–875. https://doi.org/10.3109/14756360903489581
BibTeX
@article{akkemik2010-75662,
author = {Akkemik, Ebru and Budak, Harun and ÇİFTÇİ, Mehmet},
title = {Effects of some drugs on human erythrocyte glucose 6-phosphate dehydrogenase: an in vitro study},
journal = {JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY},
year = {2010},
volume = {25},
number = {6},
pages = {871--875},
issn = {1475-6366},
doi = {10.3109/14756360903489581},
url = {https://akademik.atauni.edu.tr/yayinlar/75662-effects-of-some-drugs-on-human-erythrocyte-glucose-6-phosphate-dehydrogenase-an}
}