Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout

Ceyhun, Saltuk Buğrahan; SENTURK, Murat; EKINCI, Deniz; Erdoğan, Orhan; Çiltaş, Abdulkadir; Kocaman, Esat Mahmut

doi:10.1016/j.cbpc.2010.04.008

Makale Tam Makale Uluslararası 2010

Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout

Saltuk Buğrahan Ceyhun, Murat SENTURK, Deniz EKINCI, Orhan Erdoğan, Abdulkadir Çiltaş, Esat Mahmut Kocaman

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY Cilt 152 · Sayı 2 · ss. 215–223 · 01.08.2010

DOI ile aç

Özet

The current research aims to determine alterations in gene expression and enzymatic activity of fish antioxidant metabolism in response to pesticide administration. To this end, three different deltamethrin concentrations (0.25, 1, 2.5 mu g/L) were administrated to rainbow trout (Oncorhynchus mykiss) at different time intervals (6, 12, 24, 48 and 72 h) in order to observe the influences of the pesticide on the activity of glutathione reductase, glucose 6-phosphate dehydrogenase, 6-ghosphogluconate dehydrogenase, and the expression of Hsp70 gene. We observed that the activities of the enzymes decreased with increasing deltamethrin concentrations and exposure time. The pesticide had more inhibitory effects on gill enzymes than those of muscle, liver and kidney.. In addition, we detected that deltamethrin increased the expression of the stress-related protein Hsp70 with significant fold-chance values. The efficiency rate was 96.4% which is equal to 1.96 calculated via conversion formula used to calculate fold-chance value. We conclude that deltamethrin causes oxidative stress in fish both at protein and mRNA levels. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.

Anahtar kelimeler

stress oxidative stress IN-VITRO LIPID-PEROXIDATION GENE-EXPRESSION Glutathione ENZYME-ACTIVITY Deltamethrin HSP70 6-PHOSPHOGLUCONATE DEHYDROGENASE Oncorhynchus mykiss ERYTHROCYTE GLUTATHIONE-REDUCTASE Pentose phosphate GLUCOSE-6-PHOSPHATE-DEHYDROGENASE ENZYME HEPATIC HEAT-SHOCK-PROTEIN-70 METHYL PARATHION

Atıf için künye

Ceyhun, S. B., SENTURK, M., EKINCI, D., Erdoğan, O., Çiltaş, A., & Kocaman, E. M. (2010). Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 152(2), 215–223. https://doi.org/10.1016/j.cbpc.2010.04.008

BibTeX

@article{ceyhun2010-75448,
  author = {Ceyhun, Saltuk Buğrahan and SENTURK, Murat and EKINCI, Deniz and Erdoğan, Orhan and Çiltaş, Abdulkadir and Kocaman, Esat Mahmut},
  title = {Deltamethrin attenuates antioxidant defense system and induces the expression of heat shock protein 70 in rainbow trout},
  journal = {COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY},
  year = {2010},
  volume = {152},
  number = {2},
  pages = {215--223},
  issn = {1532-0456},
  doi = {10.1016/j.cbpc.2010.04.008},
  url = {https://akademik.atauni.edu.tr/yayinlar/75448-deltamethrin-attenuates-antioxidant-defense-system-and-induces-the-expression-of}
}