In vitro and in vivo effects of iron on the expression and activity of glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and glutathione reductase in rat spleen

Gönül Baltacı, Nurdan; Guler, C; Ceylan, Hamid; Kalın, Sn; Adem, S; Kocpınar, Enver Fehim; Erdoğan, Orhan; Budak, Harun

doi:10.1002/jbt.22229

Makale Tam Makale Uluslararası 2019

In vitro and in vivo effects of iron on the expression and activity of glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and glutathione reductase in rat spleen

Nurdan Gönül Baltacı, C Guler, Hamid Ceylan, Sn Kalın, S Adem, Enver Fehim Kocpınar, Orhan Erdoğan, Harun Budak

JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY Sayı 1 · 01.01.2019

DOI ile aç

Özet

Iron is an indispensable element for vital activities in almost all living organisms. It is also a cofactor for many proteins, enzymes, and other essential complex biochemical processes. Therefore, iron trafficking is firmly regulated by Hepcidin (Hamp), which is regarded as the marker for iron accumulation. The disruption of iron homeostasis leads to oxidative stress that causes various human diseases, but this mechanism is still unclear. The aim of this study is to provide a better in vivo and in vitro understanding of how long-term iron overload affects the gene expression and activities of some antioxidant enzymes, such as glucose 6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), and glutathione reductase (GR) in the spleen. The findings of this study show that iron overload reduces the gene expression of G6pd, 6pgd, and Gr, but its actual effect was on the protein level.

Anahtar kelimeler

oxidative stress toxicity ANTIOXIDANT ENZYMES MECHANISMS EXPOSURE ENZYME-ACTIVITY PURIFICATION CHROMATOGRAPHY ENZYME PROTEINS metal ions AFFINITY MESSENGER-RNA EXPRESSION iron overload rat spleen

Sürdürülebilir Kalkınma Amaçları

SKA 3: Sağlıklı Bireyler

Atıf için künye

Gönül Baltacı, N., Guler, C., Ceylan, H., Kalın, S., Adem, S., Kocpınar, E. F., Erdoğan, O., & Budak, H. (2019). In vitro and in vivo effects of iron on the expression and activity of glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and glutathione reductase in rat spleen. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY(1). https://doi.org/10.1002/jbt.22229

BibTeX

@article{gonulbaltaci2019-74937,
  author = {Gönül Baltacı, Nurdan and Guler, C and Ceylan, Hamid and Kalın, Sn and Adem, S and Kocpınar, Enver Fehim and Erdoğan, Orhan and Budak, Harun},
  title = {In vitro and in vivo effects of iron on the expression and activity of glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, and glutathione reductase in rat spleen},
  journal = {JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY},
  year = {2019},
  number = {1},
  issn = {1095-6670},
  doi = {10.1002/jbt.22229},
  url = {https://akademik.atauni.edu.tr/yayinlar/74937-in-vitro-and-in-vivo-effects-of-iron-on-the-expression-and-activity-of-glucose-6}
}