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Makale Tam Makale Uluslararası 2015

Neurotoxic effects of nickel chloride in the rainbow trout brain: Assessment of c-Fos activity, antioxidant responses, acetylcholinesterase activity, and histopathological changes

Ahmet Topal, Muhammed Atamanalp, Ertan Oruc, Mesut Bünyami HALICI, Melda ŞİŞECİOĞLU, Hüseyin Serkan Erol, Arzu GERGIT, Bahar YILMAZ

Fish Physiology and Biochemistry Cilt 41 · Sayı 3 · ss. 625–634 · 01.06.2015
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Özet

The aim of this study was to determine the biochemical, immunohistochemical, and histopathological effects of nickel chloride (Ni) in the rainbow trout brain. Fish were exposed to Ni concentrations (1 mg/L and 2 mg/L) for 21 days. At the end of the experimental period, brain tissues were taken from all fish for c-Fos activity and histopathological examination and determination of acetylcholinesterase (AChE), superoxide dismutase (SOD), catalase (CAT) enzyme activities, lipid peroxidation (LPO), and glutathione (GSH) levels. Our results showed that Ni treatment caused a significant increase in the brain SOD activity and in LPO and GSH levels (p < 0.05), but it significantly decreased AChE and CAT enzyme activities (p < 0.05). Strong induction in c-Fos was observed in some cerebral and cerebellar regions of fish exposed to Ni concentrations when compared with the control group. However, c-Fos activity was decreased in necrotic Purkinje cells. Brain tissues were characterized by demyelination and necrotic changes. These results suggested that Ni treatment causes oxidative stress, changes in c-Fos activity, and histopathological damage in the fish brain.

Anahtar kelimeler

oxidative stress IN-VITRO LIPID-PEROXIDATION toxicity alpha lipoic acid BRAIN liver Oxidative stress biomarkers FISH Nickel Hematological parameters C-FOS FRESH-WATER FISH MESSENGER-RNA EXPRESSION TERM EXPOSURE

Atıf için künye

Topal, A., Atamanalp, M., Oruc, E., HALICI, M. B., ŞİŞECİOĞLU, M., Erol, H. S., GERGIT, A., & YILMAZ, B. (2015). Neurotoxic effects of nickel chloride in the rainbow trout brain: Assessment of c-Fos activity, antioxidant responses, acetylcholinesterase activity, and histopathological changes. Fish Physiology and Biochemistry, 41(3), 625–634. https://doi.org/10.1007/s10695-015-0033-1

BibTeX 
@article{topal2015-74637,
  author = {Topal, Ahmet and Atamanalp, Muhammed and Oruc, Ertan and HALICI, Mesut Bünyami and ŞİŞECİOĞLU, Melda and Erol, Hüseyin Serkan and GERGIT, Arzu and YILMAZ, Bahar},
  title = {Neurotoxic effects of nickel chloride in the rainbow trout brain: Assessment of c-Fos activity, antioxidant responses, acetylcholinesterase activity, and histopathological changes},
  journal = {Fish Physiology and Biochemistry},
  year = {2015},
  volume = {41},
  number = {3},
  pages = {625--634},
  issn = {0920-1742},
  doi = {10.1007/s10695-015-0033-1},
  url = {https://akademik.atauni.edu.tr/yayinlar/74637-neurotoxic-effects-of-nickel-chloride-in-the-rainbow-trout-brain-assessment-of-c}
}