CHEMICAL PROFILE OF Ficus carica L. LEAF METHANOL EXTRACT AND ITS EFFECT ON CELL VIABILITY
Özet
Ficus carica L. is one of the oldest known cultivated plants belonging to the Moraceae family.
It constitutes one of the largest angiosperm genera, with over 800 species of shrubs, epiphytes
and trees cultivated in various subtropical regions of the world. F. carica (FC) leaves have been
used throughout history for both tea and traditional healing purposes. This study investigates
FC leaf methanol extract content and its proliferation and cell viability properties on Human
dermal fibroblast (HDF) cell line. F. carica leaves were collected from Bursa province of
Turkey, dried, powdered and extracted. Firstly, several components of the extract were
identified using Gas Chromatography-Mass Spectrometry (GC-MS) and secondly, HDF cell
viability and proliferative effects (WST-8 analysis) were evaluated. Fourteen chemical
components in FC leaf methanol extract were identified by GC-MS. As a result of GC-MS
analysis, it was determined that the FC leaf methanol extract contained α-tocopherol (28.181%)
and sucrose (22.600%); other components were β-sitosterol (13.607%), squalene (6.891%),
diisooctyl phthalate (4.479%), bis(2-ethylhexyl) isophthalate (4.045%), phytol (3.803%), 1,3-
distearin (3.426%), ursolic acid methyl ester (3.355%), hexanedioic acid bis(2-ethylhexyl) ester
(2.625%), β-amyrin (2.366%), heptacosane (2.154%), 5β-cholestan-3-one (1.989%)
andömüritose (0.479%). When the methanol extract of FC leaf was applied to HDF cell line, it
was observed that cell viability decreased by 12.855% at 500 µg/mL concentration. On the
other hand, it had a significant effect on cell viability and proliferation at concentrations between 15.625-250 µg/mL. As a result of these findings, it was concluded that FC leaf
methanol extract increased cell proliferation and viability.
Atıf için künye
Uğuz Bayrakçeken, H., Avcı, B., Sulumer, A. N., Palabıyık, E., Aşkın, H., Çelik, M., & Atmaca, U. (2024). CHEMICAL PROFILE OF Ficus carica L. LEAF METHANOL EXTRACT AND ITS EFFECT ON CELL VIABILITY. ISARC INTERNATIONAL SCIENCE AND ART RESEARCH CENTER, Trabzon (ss. 453–455).
BibTeX
@inproceedings{uguzbayrakceken2024-71802,
author = {Uğuz Bayrakçeken, Handan and Avcı, Bahri and Sulumer, Ayşe Nurseli and Palabıyık, Esra and Aşkın, Hakan and Çelik, Murat and Atmaca, Ufuk},
title = {CHEMICAL PROFILE OF Ficus carica L. LEAF METHANOL EXTRACT AND ITS EFFECT ON CELL VIABILITY},
booktitle = {ISARC INTERNATIONAL SCIENCE AND ART RESEARCH CENTER},
address = {Trabzon},
year = {2024},
pages = {453--455},
url = {https://akademik.atauni.edu.tr/yayinlar/71802-chemical-profile-of-ficus-carica-l-leaf-methanol-extract-and-its-effect-on-cell}
}