Determination of neurotoxic agents as markers of common vetch adulteration in lentil by LC-MS/MS

GHASEMI, Nesa; Seçen, Hasan; YILMAZ, Hasibe; BINICI, Burcu; GOREN, Ahmet C.

doi:10.1016/j.foodchem.2016.11.079

Makale Tam Makale Uluslararası 2017

Determination of neurotoxic agents as markers of common vetch adulteration in lentil by LC-MS/MS

Nesa GHASEMI, Hasan Seçen, Hasibe YILMAZ, Burcu BINICI, Ahmet C. GOREN

FOOD CHEMISTRY Cilt 221 · ss. 2005–2009 · 15.04.2017

DOI ile aç

Özet

Vicia sativa (common vetch), which contains beta-cyanoalanine (BCA) and c-glutamyl beta-cyanoalanine (cGBCA) is used for adulteration of lentil and have neuro toxic effects on people and animals. Therefore the determination of BCA and gamma GBCA analytes in accurate and reliable manner has high importance for human and animal health. LC-IDMS/MS method has been developed and validated for quantitative analyses of beta-cyanoalanine and C-13 labeled BCA is used as an internal standard in gamma GBCA analysis. In order to validate the method, linearity, recovery, precision (repeatability), intermediate precision, limit of detection, and limit of quantification parameters were investigated. The correlation coefficient was found to be greater than 0.99 for both analytes. The recoveriess were determined as 95.8% for BCA and 97.4% for gamma GBCA. The relative expanded measurement uncertainties of beta-cyanoalanine and gamma-glutamyl-beta-cyanoalanine were obtained as 4.6% and 5.8%, respectively with the coverage factor, k, is 2 and at 95% confidence level. (C) 2016 Elsevier Ltd. All rights reserved.

Anahtar kelimeler

LC-MS/MS Method validation uncertainty beta-Cyano-L-alanine Lentil Common vetch

Sürdürülebilir Kalkınma Amaçları

SKA 3: Sağlıklı Bireyler
SKA 16: Barış, Adalet ve Güçlü Kurumlar

Atıf için künye

GHASEMI, N., Seçen, H., YILMAZ, H., BINICI, B., & GOREN, A. C. (2017). Determination of neurotoxic agents as markers of common vetch adulteration in lentil by LC-MS/MS. FOOD CHEMISTRY, 221, 2005–2009. https://doi.org/10.1016/j.foodchem.2016.11.079

BibTeX

@article{ghasemi2017-71392,
  author = {GHASEMI, Nesa and Seçen, Hasan and YILMAZ, Hasibe and BINICI, Burcu and GOREN, Ahmet C.},
  title = {Determination of neurotoxic agents as markers of common vetch adulteration in lentil by LC-MS/MS},
  journal = {FOOD CHEMISTRY},
  year = {2017},
  volume = {221},
  pages = {2005--2009},
  issn = {0308-8146},
  doi = {10.1016/j.foodchem.2016.11.079},
  url = {https://akademik.atauni.edu.tr/yayinlar/71392-determination-of-neurotoxic-agents-as-markers-of-common-vetch-adulteration-in}
}