Characterization and anions inhibition studies of an α-carbonic anhydrase from the teleost fish Dicentrarchus labrax.

Ekinci, Deniz; Ceyhun, Saltuk Buğrahan; SENTURK, Murat; Kılıç, Deryanur; Küfrevioğlu, Ömer İrfan; SUPURAN, Claudiu T.

doi:10.1016/j.bmc.2010.12.033

Makale Tam Makale Uluslararası 2011

Characterization and anions inhibition studies of an α-carbonic anhydrase from the teleost fish Dicentrarchus labrax.

Deniz Ekinci, Saltuk Buğrahan Ceyhun, Murat SENTURK, Deryanur Kılıç, Ömer İrfan Küfrevioğlu, Claudiu T. SUPURAN

BIOORGANIC & MEDICINAL CHEMISTRY Cilt 19 · Sayı 2 · ss. 744 · 15.01.2011

DOI ile aç

Özet

Carbonic anhydrase (CA; EC 4.2.1.1) was purified from the gill of the teleost fish Dicentrarchus labrax (European seabass). The purification procedure consisted of a single step affinity chromatography on Sepharose 4B-tyrosine-sulfanilamide. The enzyme was purified 84.9-fold with a yield of 58%, and a specific activity of 838.9 U/mg proteins. It has an optimum pH at 8.0; an optimum temperature at 10 degrees C. The kinetic parameters of this enzyme were determined for its esterase activity, with 4-nitrophenyl acetate (NPA) as substrate. The following anions, H2NSO3-, I , SCN , NO3- , NO2- , N-3(-) , Br , Cl , SO42- , and F showed inhibitory effects on the enzyme. Sulfamic acid, iodide, and thiocyanate exhibited the strongest inhibitory action, in the micromolar range (K(i)s of 87-187 mu M). NO3- , NO2- and N-3(-) were moderate inhibitors, whereas other anions showed only weak actions. All tested anions inhibited the enzyme in a competitive manner. Our findings indicate that these anions inhibit the fish enzyme in a similar manner to other a-CAs from mammals investigated earlier, but the susceptibility to various anions differs significantly between the fish and mammalian CAs. (C) 2010 Elsevier Ltd. All rights reserved.

Anahtar kelimeler

IN-VITRO carbonic anhydrase WATER ESTERASE-ACTIVITIES ESTERASE ISOZYME-I teleost fish FRESH Alpha-class Anion inhibitor Sulfamic acid Thiocyanate OPERCULAR EPITHELIUM TRANSMEMBRANE XIII

Atıf için künye

Ekinci, D., Ceyhun, S. B., SENTURK, M., Kılıç, D., Küfrevioğlu, Ö. İ., & SUPURAN, C. T. (2011). Characterization and anions inhibition studies of an α-carbonic anhydrase from the teleost fish Dicentrarchus labrax. BIOORGANIC & MEDICINAL CHEMISTRY, 19(2), 744. https://doi.org/10.1016/j.bmc.2010.12.033

BibTeX

@article{ekinci2011-68811,
  author = {Ekinci, Deniz and Ceyhun, Saltuk Buğrahan and SENTURK, Murat and Kılıç, Deryanur and Küfrevioğlu, Ömer İrfan and SUPURAN, Claudiu T.},
  title = {Characterization and anions inhibition studies of an α-carbonic anhydrase from the teleost fish Dicentrarchus labrax.},
  journal = {BIOORGANIC & MEDICINAL CHEMISTRY},
  year = {2011},
  volume = {19},
  number = {2},
  pages = {744},
  issn = {0968-0896},
  doi = {10.1016/j.bmc.2010.12.033},
  url = {https://akademik.atauni.edu.tr/yayinlar/68811-characterization-and-anions-inhibition-studies-of-an-a-carbonic-anhydrase-from}
}