The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase

BEYDEMIR, S; KULACOGLU, DN; CIFTCI, M; Küfrevioğlu, Ömer İrfan

Makale Tam Makale Uluslararası 2003

The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase

S BEYDEMIR, DN KULACOGLU, M CIFTCI, Ömer İrfan Küfrevioğlu

TURKISH JOURNAL OF CHEMISTRY Cilt 27 · Sayı 5 · ss. 601–608 · 01.01.2003

Özet

Glucose-6-phosphate dehydrogenase (G6PD) enzyme was purified from sheep lenses, and the in vitro effects of dexamethasone on the enzyme's activity were investigated. Sheep lens glucose-6-phosphate dehydrogenase was purified 10,000-fold by using ammonium sulphate fractionation and 2',5'-ADP-Sepharose 4B affinity gel chromatography for a yield of 83.8% and a specific activity of 7.6 EU/mg protein. Enzyme activity was determined by Beutler's method. Dexamethasone strongly inhibited the enzyme under in vitro conditions. The I-50 value of the dexamethasone was 3.05 mM.

Anahtar kelimeler

INFLAMMATION superoxide dismutase DEFICIENCY Glutathione PURIFICATION PROTEIN GLUCOSE-6-PHOSPHATE-DEHYDROGENASE ENZYME-ACTIVITIES HUMAN-ERYTHROCYTES sheep eye lens dexamethasone GLUCOCORTICOID-INDUCED CATARACT SARDINIA

Atıf için künye

BEYDEMIR, S., KULACOGLU, D., CIFTCI, M., & Küfrevioğlu, Ö. İ. (2003). The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase. TURKISH JOURNAL OF CHEMISTRY, 27(5), 601–608.

BibTeX

@article{beydemir2003-68807,
  author = {BEYDEMIR, S and KULACOGLU, DN and CIFTCI, M and Küfrevioğlu, Ömer İrfan},
  title = {The in vitro effects of dexamethasone on sheep lens glucose-6-phosphate dehydrogenase},
  journal = {TURKISH JOURNAL OF CHEMISTRY},
  year = {2003},
  volume = {27},
  number = {5},
  pages = {601--608},
  issn = {1300-0527},
  url = {https://akademik.atauni.edu.tr/yayinlar/68807-the-in-vitro-effects-of-dexamethasone-on-sheep-lens-glucose-6-phosphate}
}