In vitro and in vivo effects of some pesticides on glucose-6-phosphate dehydrogenase enzyme activity from rainbow trout (Oncorhynchus mykiss) erythrocytes

Senturk, Murat; Ceyhun, Saltuk Buğrahan; Erdoğan, Orhan; Küfrevioğlu, Ömer İrfan

doi:10.1016/j.pestbp.2009.07.005

Makale Tam Makale Uluslararası 2009

In vitro and in vivo effects of some pesticides on glucose-6-phosphate dehydrogenase enzyme activity from rainbow trout (Oncorhynchus mykiss) erythrocytes

Murat Senturk, Saltuk Buğrahan Ceyhun, Orhan Erdoğan, Ömer İrfan Küfrevioğlu

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY Cilt 95 · Sayı 2 · ss. 95–99 · 01.10.2009

DOI ile aç

Özet

We investigated the in vitro and in vivo effects of some pesticides on rainbow trout erythrocyte glucose-6-phosphate dehydrogenase enzyme. The enzyme was purified 1691-fold with a specific activity of 16.235 U/mg protein and a yield of 63%. Cypermethrin, and propoxur inhibited glucose-6-phosphate dehydrogenase enzyme in vitro and deltamethrin inhibited both in vivo and in vitro. The obtained IC50 values for deltamethrin, cypermethrin, and propoxur were 0.63, 1.02, and 12 mM, respectively. The activity of the control was determined as 5.17 +/- 0.62 U/g Hb in in vivo studies. The enzyme activities of the groups treated with 0.25 g/L deltamethrin were measured at 6, 12, 24, 48, and 72 h, and found to be 4.32 +/- 0.47, 3.57 +/- 0.39, 3.47 +/- 0.45, 2.86 +/- 0.37, and 2.31 +/- 0.32 U/g Hb. In vivo experiments showed that deltamethrin significantly inhibited the G6PD enzyme activity after the 48th h (p < 0.05). (C) 2009 Elsevier Inc. All rights reserved.

Anahtar kelimeler

oxidative stress melatonin INHIBITION ERYTHROCYTE antibiotics PURIFICATION Deltamethrin Glutathione reductase GLUCOSE-6-PHOSPHATE-DEHYDROGENASE MALARIA FENVALERATE PESTICIDE Oncorhynchus mykiss ANESTHETICS

Atıf için künye

Senturk, M., Ceyhun, S. B., Erdoğan, O., & Küfrevioğlu, Ö. İ. (2009). In vitro and in vivo effects of some pesticides on glucose-6-phosphate dehydrogenase enzyme activity from rainbow trout (Oncorhynchus mykiss) erythrocytes. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 95(2), 95–99. https://doi.org/10.1016/j.pestbp.2009.07.005

BibTeX

@article{senturk2009-68799,
  author = {Senturk, Murat and Ceyhun, Saltuk Buğrahan and Erdoğan, Orhan and Küfrevioğlu, Ömer İrfan},
  title = {In vitro and in vivo effects of some pesticides on glucose-6-phosphate dehydrogenase enzyme activity from rainbow trout (Oncorhynchus mykiss) erythrocytes},
  journal = {PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY},
  year = {2009},
  volume = {95},
  number = {2},
  pages = {95--99},
  issn = {0048-3575},
  doi = {10.1016/j.pestbp.2009.07.005},
  url = {https://akademik.atauni.edu.tr/yayinlar/68799-in-vitro-and-in-vivo-effects-of-some-pesticides-on-glucose-6-phosphate}
}