Purification of Polyphenol Oxidase from Potato and Investigation of the Inhibitory Effects of Phenolic Acids on Enzyme Activity
Özet
Background: Polyphenol Oxidase (PPO) belongs to the oxidoreductase enzyme family. Methods: Here, PPO was purified from potato using Sepharose 4B-L-tyrosine-p-aminobenmic acid affinity chromatography. It determined the interactions between some phenolic acids and the enzyme. Results: The enzyme was obtained with a specific activity of 15333.33 EU/mg protein and 7.87-fold purification. It was found that phenolic acids exhibited inhibitory properties for PPO. The IC50 values of the phenolic acids were found in the range of 0.36-2.12 mM, and their K-i values were found in the range of 0.28 +/- 0.07-1.72 +/- 0.32 mM. It was determined that all studied compounds displayed a competitive inhibition effect. Among these compounds, 3-hydroxybenzoic acid was found to be the most effective PPO inhibitor (K-i: 0.28 +/- 0.07 mM). Conclusion: Investigating the inhibition kinetics of the enzyme will simplify the testing of PPO inhibitor candidates.
Anahtar kelimeler
Atıf için künye
Bayrak, S., Ozturk, C., Demir, Y., Alim, Z., & Küfrevioğlu, Ö. İ. (2020). Purification of Polyphenol Oxidase from Potato and Investigation of the Inhibitory Effects of Phenolic Acids on Enzyme Activity. PROTEIN AND PEPTIDE LETTERS(3), 187–192. https://doi.org/10.2174/0929866526666191002142301
BibTeX
@article{bayrak2020-68774,
author = {Bayrak, SONGÜL and Ozturk, Cansu and Demir, Yeliz and Alim, Zuhal and Küfrevioğlu, Ömer İrfan},
title = {Purification of Polyphenol Oxidase from Potato and Investigation of the Inhibitory Effects of Phenolic Acids on Enzyme Activity},
journal = {PROTEIN AND PEPTIDE LETTERS},
year = {2020},
number = {3},
pages = {187--192},
issn = {0929-8665},
doi = {10.2174/0929866526666191002142301},
url = {https://akademik.atauni.edu.tr/yayinlar/68774-purification-of-polyphenol-oxidase-from-potato-and-investigation-of-the}
}