Apocarbonic anhydrase-enzymatic determination of zinc in fruit juices

In this study, a new enzymatic method for determining trace amount of zinc was employed on ten different fruit juices. The basis of the enzymatic method is that apocarbonic anhydrase regains its activity in proportion to the Zn2+ existing in a solution. Thus, the more activated the enzyme is in the medium, the higher the Zn2+ concentration in the solution will be. For this purpose, bovine carbonic anhydrase was purified from erythrocyte hemolisate by affinity chromatography. The Zn2+ bound to the enzyme was removed by of the purified enzyme against the solution of pyridine-2-6-dicarboxylic acid. Thus 100% purified enzyme was obtained.