In vitro and in vivo effects of some pesticides on carbonic anhydrase enzyme from rainbow trout (Oncorhynchus mykiss) gills

Ceyhun, Saltuk Buğrahan; Senturk, Murat; Erdoğan, Orhan; Küfrevioğlu, Ömer İrfan

doi:10.1016/j.pestbp.2010.01.003

Makale Tam Makale Uluslararası 2010

In vitro and in vivo effects of some pesticides on carbonic anhydrase enzyme from rainbow trout (Oncorhynchus mykiss) gills

Saltuk Buğrahan Ceyhun, Murat Senturk, Orhan Erdoğan, Ömer İrfan Küfrevioğlu

PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY Cilt 97 · Sayı 3 · ss. 177–181 · 01.07.2010

DOI ile aç

Özet

Here we investigated the in vitro and in vivo effects of the pesticides. deltamethrin, diazinon, propoxur and cypermethrin, on the activity of rainbow trout (it) gill carbonic anhydrase (CA). The enzyme was purified from rainbow trout gills using Sepharose 4B-aniline-sulfanilamide affinity chromatography method. The overall purification was approx. 214-fold. SDS-polyacrylamide gel electrophoresis showed a single band corresponding to a molecular weight of approx. 29 kDa. The four pesticides dose-dependently inhibited in vitro CA activity. IC50 values for deltamethrin, diazinon, propoxur and cypermethrin were 0.137, 0.267, 0.420 and 0.460 mu M, respectively. In vitro results showed that pesticides inhibit rtCA activity with rank order of deltamethrin > diazinon > propoxur > cypermethrin. Besides, in vivo studies of deltamethrin were performed on CA activity of rainbow trout gill. rtCA was significantly inhibited at three concentrations (0.25, 1.0 and 2.5 mu g/L) at 24 and 48 h. (C) 2010 Elsevier Inc. All rights reserved.

Anahtar kelimeler

carbonic anhydrase INHIBITION liver PURIFICATION WATER Deltamethrin FISH metal ions KINETIC-PROPERTIES gill PESTICIDE Oncorhynchus mykiss ERYTHROCYTE GLUTATHIONE-REDUCTASE HISTOCHEMICAL-LOCALIZATION FRESH

Atıf için künye

Ceyhun, S. B., Senturk, M., Erdoğan, O., & Küfrevioğlu, Ö. İ. (2010). In vitro and in vivo effects of some pesticides on carbonic anhydrase enzyme from rainbow trout (Oncorhynchus mykiss) gills. PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY, 97(3), 177–181. https://doi.org/10.1016/j.pestbp.2010.01.003

BibTeX

@article{ceyhun2010-68695,
  author = {Ceyhun, Saltuk Buğrahan and Senturk, Murat and Erdoğan, Orhan and Küfrevioğlu, Ömer İrfan},
  title = {In vitro and in vivo effects of some pesticides on carbonic anhydrase enzyme from rainbow trout (Oncorhynchus mykiss) gills},
  journal = {PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY},
  year = {2010},
  volume = {97},
  number = {3},
  pages = {177--181},
  issn = {0048-3575},
  doi = {10.1016/j.pestbp.2010.01.003},
  url = {https://akademik.atauni.edu.tr/yayinlar/68695-in-vitro-and-in-vivo-effects-of-some-pesticides-on-carbonic-anhydrase-enzyme}
}