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Makale Tam Makale Uluslararası 2018

Inhibitory properties of some heavy metals on carbonic anhydrase I and II isozymes activities purified from Van Lake fish (Chalcalburnus Tarichi) gill

Muslum Kuzu, Veysel Comakli, Ebru Akkemik, Mehmet Ciftci, Ömer İrfan Küfrevioğlu

Fish Physiology and Biochemistry Cilt 44 · Sayı 4 · ss. 1119–1125 · 01.08.2018
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Özet

In this study, CA I and II isoenzymes were purified from Van Lake fish gills by using Sepharose-4B-L-tyrosine-sulfanilamide affinity chromatography and to determine the effects of some metals on the enzyme activities. For purified CA I isoenzyme, yield, specific activity, and purification fold were obtained as 42.07%, 4948.12 EU/mg protein, and 116.61 and for CA II isoenzyme, 7%, 1798.56 EU/mg protein, and 42.38 respectively. Activity of CA was determined by measuring "CO2-hydratase activity". Purity control was checked by SDS-PAGE. In vitro inhibitory effect of Cu2+, Ag+, Cd2+, Ni2+ metal ions, and arsenic (V) oxide were also examined for both isozymes activities. Whereas Cu2+, Ag+, Cd2+, and Ni2+ ions showed inhibitory effects on both isozymes, arsenic (V) oxide showed activation effect. IC50 values were calculated by drawing activity %-[I] graphs for metal ions exhibiting inhibitory effects. IC50 values were determined as 3.39, 6.38, 13.52, and 206 mu M for CA I isozyme and 6.16, 20.29, 46, and 223 mu M for CA II isozyme respectively.

Anahtar kelimeler

carbonic anhydrase INHIBITION liver TROUT ONCORHYNCHUS-MYKISS ENZYME-ACTIVITY PURIFICATION VITRO Rainbow trout IONS Heavy metal KINETIC-PROPERTIES CADMIUM Salmo trutta Labrax

Atıf için künye

Kuzu, M., Comakli, V., Akkemik, E., Ciftci, M., & Küfrevioğlu, Ö. İ. (2018). Inhibitory properties of some heavy metals on carbonic anhydrase I and II isozymes activities purified from Van Lake fish (Chalcalburnus Tarichi) gill. Fish Physiology and Biochemistry, 44(4), 1119–1125. https://doi.org/10.1007/s10695-018-0499-8

BibTeX 
@article{kuzu2018-68683,
  author = {Kuzu, Muslum and Comakli, Veysel and Akkemik, Ebru and Ciftci, Mehmet and Küfrevioğlu, Ömer İrfan},
  title = {Inhibitory properties of some heavy metals on carbonic anhydrase I and II isozymes activities purified from Van Lake fish (Chalcalburnus Tarichi) gill},
  journal = {Fish Physiology and Biochemistry},
  year = {2018},
  volume = {44},
  number = {4},
  pages = {1119--1125},
  issn = {0920-1742},
  doi = {10.1007/s10695-018-0499-8},
  url = {https://akademik.atauni.edu.tr/yayinlar/68683-inhibitory-properties-of-some-heavy-metals-on-carbonic-anhydrase-i-and-ii}
}