Heavy metal ion inhibition studies of human, sheep and fish alpha-carbonic anhydrases
Özet
Carbonic anhydrases (CAs, EC 4.2.1.1) were purified from sheep kidney (sCA IV), from the liver of the teleost fish Dicentrarchus labrax (dCA) and from human erythrocytes (hCA I and hCA II). The purification procedure consisted of a single step affinity chromatography on Sepharose 4B-tyrosine-sulfanilamide. The kinetic parameters of these enzymes were determined for their esterase activity with 4-nitrophenyl acetate as substrate. The following metal ions, Pb2+, Co2+, Hg2+, Cd2+, Zn2+, Se2+, Cu2+, Al3+ and Mn3+ showed inhibitory effects on these enzymes. The tested metal ions inhibited these CAs competitively in the low milimolar/submillimolar range. The susceptibility to various cations inhibitors differs significantly between these vertebrate alpha-CAs and is probably due to their binding to His64 or the histidine cluster.
Anahtar kelimeler
Atıf için künye
DEMIRDAG, R., YERLIKAYA, E., SENTURK, M., Küfrevioğlu, Ö. İ., & SUPURAN, C. T. (2013). Heavy metal ion inhibition studies of human, sheep and fish alpha-carbonic anhydrases. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 28(2), 278–282. https://doi.org/10.3109/14756366.2011.640633
BibTeX
@article{demirdag2013-68669,
author = {DEMIRDAG, Ramazan and YERLIKAYA, Emrah and SENTURK, Murat and Küfrevioğlu, Ömer İrfan and SUPURAN, Claudiu T.},
title = {Heavy metal ion inhibition studies of human, sheep and fish alpha-carbonic anhydrases},
journal = {JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY},
year = {2013},
volume = {28},
number = {2},
pages = {278--282},
issn = {1475-6366},
doi = {10.3109/14756366.2011.640633},
url = {https://akademik.atauni.edu.tr/yayinlar/68669-heavy-metal-ion-inhibition-studies-of-human-sheep-and-fish-alpha-carbonic}
}