Purification of carbonic anhydrase-II from sheep liver and inhibitory effects of some heavy metals on enzyme activity
Özet
In this study; sheep carbonic anhydrase-II (SCA-II) (E.C:4.2.1.1) was purified from sheep liver and in vitro effects of heavy metals on the enzyme was examined. SCA-II isozyme was purified with about 203 purification fold, a specific activity of 2320 EU/mg-protein and a yield of 72 by using Sepharose-4B-L tyrosine-sulfanilamide affinity gel chromatography. Purity of the SCA-II enzyme was verified by SDS-PAGE technique and subunit molecular mass of the enzyme was found as 29 kDa. In addition to this, inhibitory effects of some metal ions on the enzyme were examined. In this study, sheep liver tissue was chosen; because the liver is an organ in which metal wastes of air, water and food are collected and it is easy to obtain the liver tissue. Because of the very important duties of CA enzyme on living beings, the effect of metals on the CA enzyme was investigated.
Anahtar kelimeler
Sürdürülebilir Kalkınma Amaçları
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SKA 12: Sorumlu Üretim ve Tüketim
Atıf için künye
DEMIRDAG, R., YERLIKAYA, E., & Küfrevioğlu, Ö. İ. (2012). Purification of carbonic anhydrase-II from sheep liver and inhibitory effects of some heavy metals on enzyme activity. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 27(6), 795–799. https://doi.org/10.3109/14756366.2011.615744
BibTeX
@article{demirdag2012-68657,
author = {DEMIRDAG, Ramazan and YERLIKAYA, Emrah and Küfrevioğlu, Ömer İrfan},
title = {Purification of carbonic anhydrase-II from sheep liver and inhibitory effects of some heavy metals on enzyme activity},
journal = {JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY},
year = {2012},
volume = {27},
number = {6},
pages = {795--799},
issn = {1475-6366},
doi = {10.3109/14756366.2011.615744},
url = {https://akademik.atauni.edu.tr/yayinlar/68657-purification-of-carbonic-anhydrase-ii-from-sheep-liver-and-inhibitory-effects-of}
}