ICP-MS and HPLC analyses, enzyme inhibition and antioxidant potential of Achillea schischkinii Sosn.
Özet
Achillea schischkinii Sosn. is an endemic plant species and it belongs to Asteraceae family. It is distributed widely in the Central and East Anatolia. This study was carried out for evaluation of the antioxidant activity, enzyme inhibition effect, elemental and phenolic content of A. schischkinii. Briefly, acetylcholinesterase (AChE), butyr-ylcholinesterase (BChE), alpha-glycosidase (alpha-Gly), and glutathione S-transferase (GST) enzymes were strongly inhibited by A. schischkinii. IC50 values for AChE, BChE, a-Gly, and GST enzymes were found as 19.3 mg/mL, 15.4 mg/mL, 69.3 mg/mL, and 34.7 mg/mL respectively. The antioxidant activity of the sample was evaluated by four different in vitro bioanalytical methods. Besides, the concentrations of twelve elements in A. schischkinii were analyzed by ICP-MS technique. Zn (50.6 ppm), Mn (23.0 ppm), and Cu (12.7 ppm) were found as major elements. Furthermore, catechin (20.8 mu g/mg extract), trans-ferulic acid (18.3 mu g/mg extract), and gallic acid (11.2 mu g/mg extract) were characterized as major phenolic compounds by using HPLC.
Anahtar kelimeler
Atıf için künye
Türkan, F., Atalar, M. N., Aras, A., Gülçin, İ., & Bursal, E. (2020). ICP-MS and HPLC analyses, enzyme inhibition and antioxidant potential of Achillea schischkinii Sosn. Bioorganic Chemistry, 94. https://doi.org/10.1016/j.bioorg.2019.103333
BibTeX
@article{turkan2020-68068,
author = {Türkan, Fikret and Atalar, Mehmet Nuri and Aras, Abdülmelik and Gülçin, İlhami and Bursal, Ercan},
title = {ICP-MS and HPLC analyses, enzyme inhibition and antioxidant potential of Achillea schischkinii Sosn.},
journal = {Bioorganic Chemistry},
year = {2020},
volume = {94},
issn = {0045-2068},
doi = {10.1016/j.bioorg.2019.103333},
url = {https://akademik.atauni.edu.tr/yayinlar/68068-icp-ms-and-hplc-analyses-enzyme-inhibition-and-antioxidant-potential-of-achillea}
}