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Screening of Carbonic Anhydrase, Acetylcholinesterase, Butyrylcholinesterase, and alpha-Glycosidase Enzyme Inhibition Effects and Antioxidant Activity of Coumestrol

LOKMAN DURMAZ, Adem Ertürk, MEHMET AKYÜZ, Leyla Polat Kose, Eda Mehtap Uc, Zeynebe Bingol, Ruya Saglamtas, Saleh Alwasel, İlhami Gülçin

MOLECULES Cilt 27 · Sayı 10 · 01.05.2022
DOI ile aç

Özet

Coumestrol (3,9-dihydroxy-6-benzofuran [3,2-c] chromenone) as a phytoestrogen and polyphenolic compound is a member of the Coumestans family and is quite common in plants. In this study, antiglaucoma, antidiabetic, anticholinergic, and antioxidant effects of Coumestrol were evaluated and compared with standards. To determine the antioxidant activity of coumestrol, several methods-namely N,N-dimethyl-p-phenylenediamine dihydrochloride radical (DMPD center dot+)-scavenging activity, 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulphonate) radical (ABTS(center dot+))-scavenging activity, 1,1-diphenyl-2-picrylhydrazyl radical (DPPH center dot)-scavenging activity, potassium ferric cyanide reduction ability, and cupric ion (Cu2+)-reducing activity-were performed. Butylated hydroxyanisole (BHA), Trolox, alpha-Tocopherol, and butylated hydroxytoluene (BHT) were used as the reference antioxidants for comparison. Coumestrol scavenged the DPPH radical with an IC50 value of 25.95 mu g/mL (r(2): 0.9005) while BHA, BHT, Trolox, and alpha-Tocopherol demonstrated IC50 values of 10.10, 25.95, 7.059, and 11.31 mu g/mL, respectively. When these results evaluated, Coumestrol had similar DPPH center dot-scavenging effect to BHT and lower better than Trolox, BHA and alpha-tocopherol. In addition, the inhibition effects of Coumestrol were tested against the metabolic enzymes acetylcholinesterase (AChE), butyrylcholinesterase (BChE), carbonic anhydrase II (CA II), and alpha-glycosidase, which are associated with some global diseases such as Alzheimer's disease (AD), glaucoma, and diabetes. Coumestrol exhibited K-i values of 10.25 +/- 1.94, 5.99 +/- 1.79, 25.41 +/- 1.10, and 30.56 +/- 3.36 nM towards these enzymes, respectively.

Anahtar kelimeler

ANTIOXIDANT ACTIVITY carbonic anhydrase Acetylcholinesterase Butyrylcholinesterase SULFONAMIDE DERIVATIVES ISOENZYMES I BIOLOGICAL EVALUATION phenolic compound alpha-glycosidase IN-VITRO ANTIOXIDANT PHENETHYL ESTER CAPE POLYPHENOL CONTENTS CAFFEIC ACID RADICAL SCAVENGING ACTIVITIES ANTIRADICAL ACTIVITIES ANTICHOLINERGIC ACTIVITIES coumestrol

Sürdürülebilir Kalkınma Amaçları

  • SKA 3: Sağlıklı Bireyler

Atıf için künye

DURMAZ, L., Ertürk, A., AKYÜZ, M., Kose, L. P., Uc, E. M., Bingol, Z., Saglamtas, R., Alwasel, S., & Gülçin, İ. (2022). Screening of Carbonic Anhydrase, Acetylcholinesterase, Butyrylcholinesterase, and alpha-Glycosidase Enzyme Inhibition Effects and Antioxidant Activity of Coumestrol. MOLECULES, 27(10). https://doi.org/10.3390/molecules27103091

BibTeX 
@article{durmaz2022-67987,
  author = {DURMAZ, LOKMAN and Ertürk, Adem and AKYÜZ, MEHMET and Kose, Leyla Polat and Uc, Eda Mehtap and Bingol, Zeynebe and Saglamtas, Ruya and Alwasel, Saleh and Gülçin, İlhami},
  title = {Screening of Carbonic Anhydrase, Acetylcholinesterase, Butyrylcholinesterase, and alpha-Glycosidase Enzyme Inhibition Effects and Antioxidant Activity of Coumestrol},
  journal = {MOLECULES},
  year = {2022},
  volume = {27},
  number = {10},
  issn = {1420-3049},
  doi = {10.3390/molecules27103091},
  url = {https://akademik.atauni.edu.tr/yayinlar/67987-screening-of-carbonic-anhydrase-acetylcholinesterase-butyrylcholinesterase-and}
}