Purification and Characterization of Polyphenol Oxidase from Hemsin Apple (Malus communis L.)

AYDIN, Bahar; Gülçin, İlhami; ALWASEL, Saleh H.

doi:10.1080/10942912.2015.1012725

Makale Tam Makale Uluslararası 2015

Purification and Characterization of Polyphenol Oxidase from Hemsin Apple (Malus communis L.)

Bahar AYDIN, İlhami Gülçin, Saleh H. ALWASEL

INTERNATIONAL JOURNAL OF FOOD PROPERTIES Cilt 18 · Sayı 12 · ss. 2735–2745 · 02.12.2015

DOI ile aç

Özet

The polyphenol oxidase (PPO) enzyme was purified and characterized from Hemin Apple (Malus communis L.), which was organically grown in Hemin, in the Rize province of Turkey. Enzyme (PPO) activation was determined with catechol substrate. Apples were homogenized with homogenate buffer (pH 8.5). This process was followed by precipitation with (20-80%) saturated solid (NH4)(2)SO4 and dialysis. Finally, purification with DE52-Cellulose ion-exchange and Sephadex G-25 columns was performed. Experiments were performed at an optimum pH (5.5) and optimum temperature (30-40 degrees C). The kinetic and thermal parameters Km (3.40 mM), Vmax (333.3 EU/mL.min), Ea (3.57 kcal), H (2.968 kcal/mol), Q(10) (1.33), k(cat) (24.57 min(-1)) and V-0 (7.2x10(3) mM(-1).min(-1)) were assessed. Additionally, the effects of Mg2+, Pb (2+), Fe2+, Fe3+, Cd2+, Cu2+, Zn2+, Co2+, Al3+, Mn2+ and Na+ on enzyme activity was recorded, and the IC50 values, K values and inhibition types were determined.

Anahtar kelimeler

ACID melatonin carbonic anhydrase STORAGE enzyme inhibition TROUT ONCORHYNCHUS-MYKISS ERYTHROCYTES IN-VITRO PEROXIDASE FRUITS Polyphenol oxidase GLUCOSE-6-PHOSPHATE-DEHYDROGENASE enzyme purification enzyme characterization ANHYDRASE ENZYME-ACTIVITY Hemin apple Malus communis

Atıf için künye

AYDIN, B., Gülçin, İ., & ALWASEL, S. H. (2015). Purification and Characterization of Polyphenol Oxidase from Hemsin Apple (Malus communis L.). INTERNATIONAL JOURNAL OF FOOD PROPERTIES, 18(12), 2735–2745. https://doi.org/10.1080/10942912.2015.1012725

BibTeX

@article{aydin2015-67867,
  author = {AYDIN, Bahar and Gülçin, İlhami and ALWASEL, Saleh H.},
  title = {Purification and Characterization of Polyphenol Oxidase from Hemsin Apple (Malus communis L.)},
  journal = {INTERNATIONAL JOURNAL OF FOOD PROPERTIES},
  year = {2015},
  volume = {18},
  number = {12},
  pages = {2735--2745},
  issn = {1094-2912},
  doi = {10.1080/10942912.2015.1012725},
  url = {https://akademik.atauni.edu.tr/yayinlar/67867-purification-and-characterization-of-polyphenol-oxidase-from-hemsin-apple-malus}
}