Antidiabetic and antiparasitic potentials: Inhibition effects of some natural antioxidant compounds on alpha-glycosidase, alpha-amylase and human glutathione S-transferase enzymes
Özet
The glutathione S-transferase (GST) was purified from fresh blood erythrocytes using affinity column chromatography. Also, alpha-amylase from porcine pancreas and alpha-glycosidase from Saccharomyces cerevisiae were used as target enzymes. In this study, these compounds were tested on alpha-amylase, alpha-glycosidase, and GST enzymes and demonstrated effective inhibitor compounds with K-i values in the range of 8.34-40.78 mu M against GST, and 120.53-892.36 nM against alpha-glycosidase. Additionally, the phenolic molecules were tested for the inhibition of alpha-amylase enzyme which determined effective inhibition profile with IC50 values in the range of 175.01-626.58 nM. Indeed, these molecules can be elective inhibitors of GST, alpha-glycosidase and alpha-amylase enzymes as antidiabetic and antiparasitic agents. (C) 2018 Elsevier B.V. All rights reserved.
Anahtar kelimeler
Atıf için künye
Gülçin, İ., TASLIMI, P., Aygun, A., SADEGHIAN, N., BASTEM, E., Küfrevioğlu, Ö. İ., Turkan, F., & Sen, F. (2018). Antidiabetic and antiparasitic potentials: Inhibition effects of some natural antioxidant compounds on alpha-glycosidase, alpha-amylase and human glutathione S-transferase enzymes. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 119, 741–746. https://doi.org/10.1016/j.ijbiomac.2018.08.001
BibTeX
@article{gulcin2018-67834,
author = {Gülçin, İlhami and TASLIMI, Parham and Aygun, Aywnur and SADEGHIAN, Nastaran and BASTEM, Enes and Küfrevioğlu, Ömer İrfan and Turkan, Fikret and Sen, Fatih},
title = {Antidiabetic and antiparasitic potentials: Inhibition effects of some natural antioxidant compounds on alpha-glycosidase, alpha-amylase and human glutathione S-transferase enzymes},
journal = {INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES},
year = {2018},
volume = {119},
pages = {741--746},
issn = {0141-8130},
doi = {10.1016/j.ijbiomac.2018.08.001},
url = {https://akademik.atauni.edu.tr/yayinlar/67834-antidiabetic-and-antiparasitic-potentials-inhibition-effects-of-some-natural}
}