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Makale Tam Makale Uluslararası 2016

Acetylcholinesterase and carbonic anhydrase isoenzymes I and II inhibition profiles of taxifolin

Hulya Gocer, Fevzi Topal, Meryem Topal, Murat KUCUK, Dilek Teke, İlhami Gülçin, Saleh H. ALWASEL, Claudiu T. SUPURAN

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY Cilt 31 · Sayı 3 · ss. 441–447 · 01.05.2016
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Özet

Taxifolin, also known as dihydroquercetin, is a flavonoid commonly found in plants. Carbonic anhydrase (CA, EC 4.2.1.1) plays an important role in many critical physiological events including carbon dioxide (CO2)/bicarbonate (HCO3-) respiration and pH regulation. There are 16 known CA isoforms in humans, of which human hCA isoenzymes I and II (hCA I and II) are ubiquitous cytosolic isoforms. In this study, the inhibition properties of taxifolin against the slow cytosolic isoenzyme hCA I, and the ubiquitous and dominant rapid cytosolic isoenzyme hCA II were studied. Taxifolin, as a naturally bioactive flavonoid, has a K-i of 29.2nM against hCA I, and 24.2nM against hCA II. For acetylcholinesterase enzyme (AChE) inhibition, K-i parameter of taxifolin was determined to be 16.7nM. These results clearly show that taxifolin inhibited both CA isoenzymes and AChE at the nM levels.

Anahtar kelimeler

ANTIOXIDANT ACTIVITY carbonic anhydrase Taxifolin phenolic compounds Flavonoids Acetylcholinesterase enzyme inhibition TROUT ONCORHYNCHUS-MYKISS SULFONAMIDE DERIVATIVES ISOZYMES I ISOFORMS I CAFFEIC ACID enzyme purification ANTIRADICAL ACTIVITIES VITRO ANTIOXIDANT

Atıf için künye

Gocer, H., Topal, F., Topal, M., KUCUK, M., Teke, D., Gülçin, İ., ALWASEL, S. H., & SUPURAN, C. T. (2016). Acetylcholinesterase and carbonic anhydrase isoenzymes I and II inhibition profiles of taxifolin. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 31(3), 441–447. https://doi.org/10.3109/14756366.2015.1036051

BibTeX 
@article{gocer2016-67726,
  author = {Gocer, Hulya and Topal, Fevzi and Topal, Meryem and KUCUK, Murat and Teke, Dilek and Gülçin, İlhami and ALWASEL, Saleh H. and SUPURAN, Claudiu T.},
  title = {Acetylcholinesterase and carbonic anhydrase isoenzymes I and II inhibition profiles of taxifolin},
  journal = {JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY},
  year = {2016},
  volume = {31},
  number = {3},
  pages = {441--447},
  issn = {1475-6366},
  doi = {10.3109/14756366.2015.1036051},
  url = {https://akademik.atauni.edu.tr/yayinlar/67726-acetylcholinesterase-and-carbonic-anhydrase-isoenzymes-i-and-ii-inhibition}
}