Rosmarinic acid: a potent carbonic anhydrase isoenzymes inhibitor
Özet
Rosmarinic acid is a water-soluble ester of caffeic acid and 3,4-dihydroxyphenyllactic acids, and is mainly found in plant species including Boraginaceae and Lamiaceae. In this research, we determined the inhibition property of rosmarinic acid on carbonic anhydrase isoenzymes I and II (hCA I and II) purified from human erythrocytes by using Sepharose-4B affinity column chromatography. hCA I and II isoenzymes were obtained with a yield of 57.9% and 67.2% and 76.5- and 509.3-fold purification of each isoenzyme, respectively. In order to show the purity of the isoenzymes, SDS-PAGE was performed and one band was observed. In vitro inhibition of both hCA I and II isoenzymes by rosmarinic acid using CO2-esterase activity gave Ki values of 86.0 mu M and 57.0 mu M, respectively.
Anahtar kelimeler
Atıf için künye
TOPAL, M., & Gülçin, İ. (2014). Rosmarinic acid: a potent carbonic anhydrase isoenzymes inhibitor. TURKISH JOURNAL OF CHEMISTRY, 38(5), 894–902. https://doi.org/10.3906/kim-1403-5
BibTeX
@article{topal2014-67679,
author = {TOPAL, Meryem and Gülçin, İlhami},
title = {Rosmarinic acid: a potent carbonic anhydrase isoenzymes inhibitor},
journal = {TURKISH JOURNAL OF CHEMISTRY},
year = {2014},
volume = {38},
number = {5},
pages = {894--902},
issn = {1300-0527},
doi = {10.3906/kim-1403-5},
url = {https://akademik.atauni.edu.tr/yayinlar/67679-rosmarinic-acid-a-potent-carbonic-anhydrase-isoenzymes-inhibitor}
}