Effects of some phenolic compounds on the inhibition of alpha-glycosidase enzyme-immobilized on Pluronic (R) F127 micelles: An in vitro and in silico study

Kassa, Solomon Bezabeh; Taslimi, Parham; ÖZEL, ŞAHİN; GÜR, BAHRİ; Gülçin, İlhami; Onganer, Yavuz

doi:10.1016/j.colsurfa.2021.127839

Makale Tam Makale Uluslararası 2022

Effects of some phenolic compounds on the inhibition of alpha-glycosidase enzyme-immobilized on Pluronic (R) F127 micelles: An in vitro and in silico study

Solomon Bezabeh Kassa, Parham Taslimi, ŞAHİN ÖZEL, BAHRİ GÜR, İlhami Gülçin, Yavuz Onganer

Colloids and Surfaces A: Physicochemical and Engineering Aspects Cilt 632 · 01.01.2022

DOI ile aç

Özet

This study aimed to consider the activity of immobilized alpha-glycosidase, significant enzyme defined in serum glucose adjustment, on Pluronic (R) F127 micelles and to compare the results with theoretical molecular docking studies. The phenolic molecules were used for the activity studies, which play a crucial role in the inhibition of alpha-glycosidase by providing conservation against pathogen factors. The immobilization of alpha-glycosidase on Pluronic (R) F127 micelles showed higher kinetic activity towards p-nitrophenyl-D-glucopyranoside (pNPG) than the free one. The inhibition profiles from some phenolic molecules were observed impressively with IC50 values between 20.47 and 1118.68 nM and K-i values between 18.51 and 1087.53 nM against immobilized alpha-glycosidase. In addition, the inhibition effects of the studied phenolic compounds have been tested with calculated negative binding energies from theoretical molecular docking studies. Negative binding energy values were as - 6.1 and - 8.3 kcalmol(-1) for p-coumaric acid and tetrakis compounds, respectively.

Anahtar kelimeler

STABILITY carbonic anhydrase DEGRADATION phenolic compounds α-glucosidase Acetylcholinesterase Butyrylcholinesterase Molecular docking enzyme inhibition BIOLOGICAL EVALUATION CRYSTAL-STRUCTURE alpha-glycosidase Pluronic (R) F127 BIOSENSOR Immobilization Negative binding energy

Atıf için künye

Kassa, S. B., Taslimi, P., ÖZEL, Ş., GÜR, B., Gülçin, İ., & Onganer, Y. (2022). Effects of some phenolic compounds on the inhibition of alpha-glycosidase enzyme-immobilized on Pluronic (R) F127 micelles: An in vitro and in silico study. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 632. https://doi.org/10.1016/j.colsurfa.2021.127839

BibTeX

@article{kassa2022-67603,
  author = {Kassa, Solomon Bezabeh and Taslimi, Parham and ÖZEL, ŞAHİN and GÜR, BAHRİ and Gülçin, İlhami and Onganer, Yavuz},
  title = {Effects of some phenolic compounds on the inhibition of alpha-glycosidase enzyme-immobilized on Pluronic (R) F127 micelles: An in vitro and in silico study},
  journal = {Colloids and Surfaces A: Physicochemical and Engineering Aspects},
  year = {2022},
  volume = {632},
  issn = {0927-7757},
  doi = {10.1016/j.colsurfa.2021.127839},
  url = {https://akademik.atauni.edu.tr/yayinlar/67603-effects-of-some-phenolic-compounds-on-the-inhibition-of-alpha-glycosidase-enzyme}
}