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Makale Tam Makale Uluslararası 2022

A New Affinity Matrixe Synthesized from Aminobenzohydrazide Derivatives for Purification of Lactoperoxidase Enzyme

Neslihan Macit Kavaz, Deniz Erel, Isil Nihan Korkmaz, Serpil Gerni, Nurgul Abul, SONGÜL Bayrak, ZEYNEP KÖKSAL, RAMAZAN KALIN, AYKUT ÖZTEKİN, Hasan Özdemir

ChemistrySelect Sayı 27 · 01.07.2022
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Özet

In this study, a new affinity process was developed for the purification of Lactoperoxidase with synthesized sixteen aminobenzohydrazide derivatives. For this purpose, ligands were covalently bound to CNBr-activated Sepharose-4B-L-tyrosine matrix and affinity columns were prepared, and LPO was purified in one step with high yield and purity. Among all synthesized molecules, the 4-amino-3-bromo-2-methylbenzohydrazide molecule had a high usable potential in the purification of Lactoperoxidase from mammalian milk. Lactoperoxidase was purified 411.8 times with a yield of 17.38 % from goat milk, 187.25 times with a yield of 9.72 % buffalo milk, 2772.4 times with a yield of 18.98 % from bovine milk, and 1246.65 times with a yield of 4.43 % from sheep milk. It was demonstrated for the first time that aminobenzohydrazide molecules could be used as ligands in the purification of Lactoperoxidase enzyme.

Anahtar kelimeler

SYSTEM INHIBITION Acetylcholinesterase Butyrylcholinesterase PURIFICATION PEROXIDASE GLUTATHIONE-S-TRANSFERASE Affinity chromatography PROTEINS Lactoperoxidase STEP PURIFICATION bovine milk aminobenzohidrazide mammalian milk

Sürdürülebilir Kalkınma Amaçları

  • SKA 3: Sağlıklı Bireyler

Atıf için künye

Kavaz, N. M., Erel, D., Korkmaz, I. N., Gerni, S., Abul, N., Bayrak, S., KÖKSAL, Z., KALIN, R., ÖZTEKİN, A., & Özdemir, H. (2022). A New Affinity Matrixe Synthesized from Aminobenzohydrazide Derivatives for Purification of Lactoperoxidase Enzyme. ChemistrySelect(27). https://doi.org/10.1002/slct.202200657

BibTeX 
@article{kavaz2022-67342,
  author = {Kavaz, Neslihan Macit and Erel, Deniz and Korkmaz, Isil Nihan and Gerni, Serpil and Abul, Nurgul and Bayrak, SONGÜL and KÖKSAL, ZEYNEP and KALIN, RAMAZAN and ÖZTEKİN, AYKUT and Özdemir, Hasan},
  title = {A New Affinity Matrixe Synthesized from Aminobenzohydrazide Derivatives for Purification of Lactoperoxidase Enzyme},
  journal = {ChemistrySelect},
  year = {2022},
  number = {27},
  issn = {2365-6549},
  doi = {10.1002/slct.202200657},
  url = {https://akademik.atauni.edu.tr/yayinlar/67342-a-new-affinity-matrixe-synthesized-from-aminobenzohydrazide-derivatives-for}
}