Potent Inhibitory Effects of Some Phenolic Acids on Lactoperoxidase
Özet
Lactoperoxidase (LPO) plays a key role in immune response against pathogens. In this study, we examined the effects of some phenolic acids on LPO. For this purpose, bovine milk LPO was purified 380.85-fold with a specific activity of 26.66EU/mg and overall yield of 73.33% by using Amberlite CG-50 H+ resin and CNBr-activated Sepharose-4B-l-tyrosine-sulfanilamide affinity chromatography. After purification, the in vitro effects of phenolic acids (tannic acid, 3,4-dihydroxybenzoic acid, 3,5- dihydroxybenzoic acid, chlorogenic acid, sinapic acid, 4-hydroxybenzoic acid, vanillic acid, salicylic acid, and 3-hydroxybenzoic acid) were investigated on LPO. These phenolic acids showed potent inhibitory effect on LPO. K-i values for these phenolic acids were found as 0.0129nM, 0.132M, 0.225M, 0.286M, 0.333M, 2.33M, 10.82M, 0.076mM, and 0.405mM, respectively. Sinapic acid and 4-hydroxybenzoic acid exhibited noncompetitive inhibition; 3,4-dihydroxybenzoic acid showed uncompetitive inhibition, and other phenolic acids showed competitive inhibition.
Atıf için künye
KÖKSAL, Z., Alim, Z., Beydemir, S., & Özdemir, H. (2016). Potent Inhibitory Effects of Some Phenolic Acids on Lactoperoxidase. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY, 30(11), 533–538. https://doi.org/10.1002/jbt.21819
BibTeX
@article{koksal2016-67323,
author = {KÖKSAL, ZEYNEP and Alim, Zuhal and Beydemir, Sukru and Özdemir, Hasan},
title = {Potent Inhibitory Effects of Some Phenolic Acids on Lactoperoxidase},
journal = {JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY},
year = {2016},
volume = {30},
number = {11},
pages = {533--538},
issn = {1095-6670},
doi = {10.1002/jbt.21819},
url = {https://akademik.atauni.edu.tr/yayinlar/67323-potent-inhibitory-effects-of-some-phenolic-acids-on-lactoperoxidase}
}