The effects of norepinephrine on lactoperoxidase enzyme (LPO)
Özet
Norepinephrine, a hormone and a neurotransmitter, was known as catecholamine. In the present study, we examined the inhibitory effect of norepinephrine on lactoperoxidase enzyme purified from bovine milk. Lactoperoxidase (LPO; E.C.1.11.1.7) was purified from bovine milk with three purification steps: Amberlite CG-50 resin, CM-Sephadex C-50 ion-exchange chromatography and Sephadex G-100 gel filtration chromatography, respectively. LPO was purified with a yield of 31.5%, a specific activity of 30.33 EU/mg proteins and 20.77 purification fold. To determine enzyme purity, SDS-PAGE was performed and single band was observed. The R-z (A(412)/A(280)) value for LPO was 0.9. The effect of norepinephrine on lactoperoxidase was determined using ABTS as a chromogenic substrate. The half maximal inhibitory concentration (IC50) value norepinephrine was found to be 67.2 mu M. Also, inhibition constant (K-i) for norepinephrine was found to be 62.0 mu M. Norepinephrine was found as noncompetitive inhibitor.
Anahtar kelimeler
Atıf için künye
Şişecioğlu, M., Gülçin, İ., ÇANKAYA, M., Atasever, A., & Özdemir, H. (2010). The effects of norepinephrine on lactoperoxidase enzyme (LPO). SCIENTIFIC RESEARCH AND ESSAYS, 5(11), 1351–1356.
BibTeX
@article{sisecioglu2010-67313,
author = {Şişecioğlu, Melda and Gülçin, İlhami and ÇANKAYA, MURAT and Atasever, Ali and Özdemir, Hasan},
title = {The effects of norepinephrine on lactoperoxidase enzyme (LPO)},
journal = {SCIENTIFIC RESEARCH AND ESSAYS},
year = {2010},
volume = {5},
number = {11},
pages = {1351--1356},
issn = {1992-2248},
url = {https://akademik.atauni.edu.tr/yayinlar/67313-the-effects-of-norepinephrine-on-lactoperoxidase-enzyme-lpo}
}