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Makale Tam Makale Uluslararası 2018

Inhibitory effects of selected pesticides on peroxidases purified by affinity chromatography

ZEYNEP KÖKSAL, Ramazan Kalin, İlhami Gülçin, Hasan Özdemir

INTERNATIONAL JOURNAL OF FOOD PROPERTIES Cilt 21 · Sayı 1 · ss. 385–394 · 01.01.2018
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Özet

The objective of this study was to determine the in vitro inhibition effects of seven commonly used pesticides including 2,4-d-acid dimethylamine, fenoxaprop-p-ethyl, glyphosate isopropylamine, haloxyfop-p-methyl, cypermethrin, -cyhalothrin, and dichlorvos on the peroxidase purified from turnip (Brassica rapa L.) and black radish (Raphanus sativus L.) using 4-amino benzohydrazide affinity column chromatography. The purification factors for the turnip and black radish peroxidases were found to be 263.29-fold (with a yield of 12.89%) and 36.20-fold (with a yield of 6.90%), respectively. Among these compounds, -cyhalothrin showed the strongest inhibitory effect against turnip peroxidase (K-i: 1.23 x 10(-2)+/- 0.21 x 10(-2)mM) as noncompetitive inhibition. On the other hand, cypermethrin demonstrated the highest inhibition effect against black radish peroxidase (K-i: 2.14 x 10(-2)+/- 0.08 x 10(-2)mM) as competitive inhibition.

Anahtar kelimeler

ACID carbonic anhydrase DERIVATIVES Acetylcholinesterase Butyrylcholinesterase enzyme inhibition ENZYME GLUTATHIONE-S-TRANSFERASE ANHYDRASE ISOENZYMES I pesticides Lactoperoxidase Plant peroxidase Radish (Raphanus sativus) Turnip (Brassica rapa) STEP PURIFICATION

Atıf için künye

KÖKSAL, Z., Kalin, R., Gülçin, İ., & Özdemir, H. (2018). Inhibitory effects of selected pesticides on peroxidases purified by affinity chromatography. INTERNATIONAL JOURNAL OF FOOD PROPERTIES, 21(1), 385–394. https://doi.org/10.1080/10942912.2018.1424197

BibTeX 
@article{koksal2018-67304,
  author = {KÖKSAL, ZEYNEP and Kalin, Ramazan and Gülçin, İlhami and Özdemir, Hasan},
  title = {Inhibitory effects of selected pesticides on peroxidases purified by affinity chromatography},
  journal = {INTERNATIONAL JOURNAL OF FOOD PROPERTIES},
  year = {2018},
  volume = {21},
  number = {1},
  pages = {385--394},
  issn = {1094-2912},
  doi = {10.1080/10942912.2018.1424197},
  url = {https://akademik.atauni.edu.tr/yayinlar/67304-inhibitory-effects-of-selected-pesticides-on-peroxidases-purified-by-affinity}
}