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Makale Tam Makale Uluslararası 2006

In vitro effects of some drugs on catalase purified from human skin

Sayit ALTIKAT, Abdulkadir COBAN, Mehmet Ciftci, Hasan Özdemir

JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY Cilt 21 · Sayı 2 · ss. 231–234 · 01.04.2006
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Özet

Catalase enzyme (H2O2: oxidoreductase; E.C. 1.11.1.6) was purified from human skin homogenate using ammonium sulfate precipitation and DEAE-Sephadex A50 ion exchange chromatography at 4 degrees C and some characteristics of the enzyme were investigated. The human skin enzyme, having a specific activity of 1354.5 EU/mg proteins was purified with a yield of 43.13% and 1110-fold. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed a single band for the enzyme. Inhibition by piroxicam, ketoprofen, diclofenac sodium, sulfamethoxazole and nidazole occurred with I-50 values of 0.414 1.29, 1.8, 3.83, and 8.64 mM, respectively.

Anahtar kelimeler

stress VIVO INHIBITION drug ENZYME-ACTIVITY PURIFICATION CATALASE GLUCOSE-6-PHOSPHATE-DEHYDROGENASE ERYTHROCYTES human skin

Atıf için künye

ALTIKAT, S., COBAN, A., Ciftci, M., & Özdemir, H. (2006). In vitro effects of some drugs on catalase purified from human skin. JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY, 21(2), 231–234. https://doi.org/10.1080/14756360500483453

BibTeX 
@article{altikat2006-67303,
  author = {ALTIKAT, Sayit and COBAN, Abdulkadir and Ciftci, Mehmet and Özdemir, Hasan},
  title = {In vitro effects of some drugs on catalase purified from human skin},
  journal = {JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY},
  year = {2006},
  volume = {21},
  number = {2},
  pages = {231--234},
  issn = {1475-6366},
  doi = {10.1080/14756360500483453},
  url = {https://akademik.atauni.edu.tr/yayinlar/67303-in-vitro-effects-of-some-drugs-on-catalase-purified-from-human-skin}
}