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Makale Tam Makale Uluslararası 2012

Purification and characterization of glutathione S transferase from turkey liver and inhibition effects of some metal ions on enzyme activity

EBRU AKKEMİK, Pınar Güller, BAYINDIR AYŞEGÜL, Harun Budak, MEHMET ÇİFTCİ

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY Cilt 34 · Sayı 3 · ss. 888–894 · 01.11.2012
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Özet

The glutathione S-transferases (EC 2.5.1.18) were purified and characterized from turkey liver for the first time. The enzyme was purified 252.7-fold with a yield of 45%, with a specific activity of 164.31 U/mg from turkey liver. The purity of the enzyme was determined by SDS-PAGE and showed two bands nearly 26 kDa and 24 kDa on the gel. The native molecular mass of the enzyme was found to be approximately 53 kDa by Sephadex G-100 gel filtration chromatography. Optimal pH, stable pH, optimal temperature, optimum ionic strength, K-m and V-max values for GSH and CDNB were also determined for the enzyme as 7.3, 8.5, 50 degrees C, 600 mM, 0.154 mM, 0.380 mM, 1.803 EU/ml, and 2.125 EU/ml, respectively. Additionally, inhibitory effects of metal ions (Cu2+, Hg2+, Fe2+, Zn2+, Ag+, Mg2+, Ni2+, and Mn2+) were examined the enzyme's activity in vitro by performing Lineweaver-Burk graphs and plotting activity% vs., respectively. (C) 2012 Elsevier B.V. All rights reserved.

Anahtar kelimeler

DISEASE oxidative stress RISK toxicity INHIBITION MECHANISMS kidney BRAIN GLUTATHIONE-S-TRANSFERASE aluminum metal ions Toxic effects HUMAN-ERYTHROCYTES MULTIPLE FORMS

Atıf için künye

AKKEMİK, E., Güller, P., AYŞEGÜL, B., Budak, H., & ÇİFTCİ, M. (2012). Purification and characterization of glutathione S transferase from turkey liver and inhibition effects of some metal ions on enzyme activity. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 34(3), 888–894. https://doi.org/10.1016/j.etap.2012.08.010

BibTeX 
@article{akkemik2012-67155,
  author = {AKKEMİK, EBRU and Güller, Pınar and AYŞEGÜL, BAYINDIR and Budak, Harun and ÇİFTCİ, MEHMET},
  title = {Purification and characterization of glutathione S transferase from turkey liver and inhibition effects of some metal ions on enzyme activity},
  journal = {ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY},
  year = {2012},
  volume = {34},
  number = {3},
  pages = {888--894},
  issn = {1382-6689},
  doi = {10.1016/j.etap.2012.08.010},
  url = {https://akademik.atauni.edu.tr/yayinlar/67155-purification-and-characterization-of-glutathione-s-transferase-from-turkey-liver}
}