Determination of genetic and epigenetic effects of glyphosate on Triticum aestivum with RAPD and CRED-RA techniques

Karadayı, Gökçe; Ağar, Güleray; Arslan Yüksel, Esra; ERTURK, Filiz Aygun

doi:10.1007/s40626-015-0039-1

Makale Tam Makale Uluslararası 2015

Determination of genetic and epigenetic effects of glyphosate on Triticum aestivum with RAPD and CRED-RA techniques

Gökçe Karadayı, Güleray Ağar, Esra Arslan Yüksel, Filiz Aygun ERTURK

Theoretical and Experimental Plant Physiology Cilt 27 · Sayı 2 · ss. 131–139 · 01.06.2015

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Özet

In this study, the most commonly used herbicide, glyphosate, was investigated for its genotoxic effects on the genome of Triticum aestivum. Five different concentrations of the herbicide were used, and alterations to DNA were measured quantitatively based on their RAPD (Randomly Amplified Polymorphic DNA) profiles. The genomic template stability (GTS%) at each concentration was evaluated, and a decrease was observed with increasing glyphosate concentration. Thus the highest concentration was concluded to be the most effective for causing alteration to DNA. Additionally, the coupled restriction enzyme digestion-random amplification (CRED-RA) technique was used to determine an epigenetic mechanism, e.g. DNA methylation. The polymorphic percentages of all concentration were calculated after herbicide applications. When in glyphosate doses compared with control group, all applications of glyphosate observed to consist of methylation. The methylation levels range from 28.3 to 73.9 % (DNA hypermethylation). In conclusion, based on the RAPD and CRED-RA results, glyphosate causes DNA alterations and methylation.

Anahtar kelimeler

stress GROWTH GENOTOXICITY L. DNA-DAMAGE WATER CYTOSINE METHYLATION wheat AMPLIFIED POLYMORPHIC DNA Daphnia magna DNA METHYLATION Glyphosate BIOSYNTHESIS INHIBITORS HERBICIDES

Atıf için künye

Karadayı, G., Ağar, G., Arslan Yüksel, E., & ERTURK, F. A. (2015). Determination of genetic and epigenetic effects of glyphosate on Triticum aestivum with RAPD and CRED-RA techniques. Theoretical and Experimental Plant Physiology, 27(2), 131–139. https://doi.org/10.1007/s40626-015-0039-1

BibTeX

@article{karadayi2015-61064,
  author = {Karadayı, Gökçe and Ağar, Güleray and Arslan Yüksel, Esra and ERTURK, Filiz Aygun},
  title = {Determination of genetic and epigenetic effects of glyphosate on Triticum aestivum with RAPD and CRED-RA techniques},
  journal = {Theoretical and Experimental Plant Physiology},
  year = {2015},
  volume = {27},
  number = {2},
  pages = {131--139},
  issn = {2197-0025},
  doi = {10.1007/s40626-015-0039-1},
  url = {https://akademik.atauni.edu.tr/yayinlar/61064-determination-of-genetic-and-epigenetic-effects-of-glyphosate-on-triticum}
}