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Makale Tam Makale Uluslararası 2009

Identification of apricot cultivars in Turkey (Prunus armeniaca L.) using RAPD markers

Sezai Ercişli, Güleray Ağar, Nalan YILDIRIM, Ahmet EŞİTKEN, Emine ORHAN

ROMANIAN BIOTECHNOLOGICAL LETTERS Cilt 14 · Sayı 4 · ss. 4582–4588 · 01.07.2009

Özet

This study was carried out to determine genetic relationships among 23 apricot cultivars from Turkey by using randomly amplified polymorphic DNA (RAPD) technology. Among the 40 decamer random primers were used for PCR reactions, 12 primers showed reproducible polymorphic patterns. These primers produced 121 bands, out of which 118 were polymorphic. UPGMA (Unweighted Pair Group Method using Arithmetic average) derived dendrogram, grouped the cultivars into one cluster and one outlier. The main cluster further divided into three subclusters. The highest dissimilarities found between Hacihaliloglu and Kabaasi cultivars (0.108). The variation detected among the closely related genotypes indicates the efficiency of RAPD markers over the morphological and isozyme markers for the identification and construction of genetic linkage maps.

Anahtar kelimeler

DIVERSITY RAPD GENETIC DIVERSITY MOLECULAR CHARACTERIZATION GERMPLASM RESOURCES Prunus armeniaca L Cluster analysis GENETIC-RELATIONSHIPS VARIETIES

Atıf için künye

Ercişli, S., Ağar, G., YILDIRIM, N., EŞİTKEN, A., & ORHAN, E. (2009). Identification of apricot cultivars in Turkey (Prunus armeniaca L.) using RAPD markers. ROMANIAN BIOTECHNOLOGICAL LETTERS, 14(4), 4582–4588.

BibTeX
@article{ercisli2009-61053,
  author = {Ercişli, Sezai and Ağar, Güleray and YILDIRIM, Nalan and EŞİTKEN, Ahmet and ORHAN, Emine},
  title = {Identification of apricot cultivars in Turkey (Prunus armeniaca L.) using RAPD markers},
  journal = {ROMANIAN BIOTECHNOLOGICAL LETTERS},
  year = {2009},
  volume = {14},
  number = {4},
  pages = {4582--4588},
  issn = {1224-5984},
  url = {https://akademik.atauni.edu.tr/yayinlar/61053-identification-of-apricot-cultivars-in-turkey-prunus-armeniaca-l-using-rapd}
}