Optimization of culture conditions for Aspergillus sojae expressing an Aspergillus fumigatus alpha-galactosidase
Özet
Using Response Surface Methodology, carbon and nitrogen sources and agitation speed for cultivation of Aspergillus sojae expressing the alpha-galactosidase gene, aglB of Aspergillus fumigatus IMI 385708 were optimized. Compared to cultivation in modified YpSs medium, cultivation in 250-mL Erlenmeyer flasks agitated at 276 rpm and containing 100 mL of optimized medium consisting of 10.5% molasses (w/v) and 1.3% NH4NO3 (w/v), 0.1% K2HPO4, and 0.005% MgSO4 center dot 7H(2)0 achieved a 4-fold increase in alpha-galactosidase production (10.4 U/mL). These results suggest the feasibility of industrial large scale production of an alpha-galactosidase known to be valuable in galactomannan modification. (C) 2011 Elsevier Ltd. All rights reserved.
Anahtar kelimeler
Atıf için künye
Gürkök, S., ÇEKMECELİOĞLU, D., & Ogel, Z. B. (2011). Optimization of culture conditions for Aspergillus sojae expressing an Aspergillus fumigatus alpha-galactosidase. BIORESOURCE TECHNOLOGY, 102(7), 4925–4929. https://doi.org/10.1016/j.biortech.2011.01.036
BibTeX
@article{gurkok2011-60086,
author = {Gürkök, Sümeyra and ÇEKMECELİOĞLU, DENİZ and Ogel, Zumrut B.},
title = {Optimization of culture conditions for Aspergillus sojae expressing an Aspergillus fumigatus alpha-galactosidase},
journal = {BIORESOURCE TECHNOLOGY},
year = {2011},
volume = {102},
number = {7},
pages = {4925--4929},
issn = {0960-8524},
doi = {10.1016/j.biortech.2011.01.036},
url = {https://akademik.atauni.edu.tr/yayinlar/60086-optimization-of-culture-conditions-for-aspergillus-sojae-expressing-an}
}