Purification and characterization of a novel extracellular, alkaline, thermoactive, and detergent-compatible lipase from Aeromonas caviae LipT51 for application in detergent industry.
Özet
<mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">Lipase</mark> producer bacterium isolated <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">from</mark> Erzurum was identified as <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">Aeromonas</mark> <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">caviae</mark> <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">LipT51</mark> (GenBank ID: MN818567.1) by 16S rDNA sequencing and conventional methods. <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">Extracellular</mark> <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">lipase</mark> was purified by ammonium sulphate precipitation, centrifugal filtration, and anion-exchange chromatography resulting <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">in</mark> 6.1-fold <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">purification</mark> with 28% final yield. Molecular weight was 31.6 kDa on SDS-PAGE. <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">Lipase</mark> was stable over <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">a</mark> broad range <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">of</mark> pH (6-11) and temperature (25-70 degrees C), and showed optimum activity at pH 9 and 60 degrees C. Km and Vmax <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">for</mark> pNPP hydrolysis were 0.88 mM and 34.2 U/mg protein, respectively. Ba2+, Ca2+, Co2+, Cu2+, Fe3+, and Mg2+ increased activity, while Mn2+, Mo2+, Ni2+, Zn2+, and other additives partially decreased. Activity and stability increased with laundry <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">detergent</mark> and slightly decreased with handwash and dishwashing detergents. <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">Alkaline</mark> and thermostable <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">lipase</mark> <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">from</mark> newly isolated <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">A</mark>. <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">caviae</mark> has been shown <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">for</mark> the first time to be remarkably compatible with laundry <mark style="margin: 0px; list-style: none; padding: 0px; font-family: "Source Sans Pro", "Noto Kufi Arabic", Arial, Helvetica, sans-serif; animation: 0.5s ease 0s 1 normal none running fadein; border-radius: 0.3125rem; color: inherit; font-size: 14px;">detergent</mark> and improve washing performance by enhanced oil-stain removal.
Anahtar kelimeler
Atıf için künye
Gürkök, S., & Özdal, M. (2021). Purification and characterization of a novel extracellular, alkaline, thermoactive, and detergent-compatible lipase from Aeromonas caviae LipT51 for application in detergent industry. PROTEIN EXPRESSION AND PURIFICATION, 180. https://doi.org/10.1016/j.pep.2021.105819
BibTeX
@article{gurkok2021-59482,
author = {Gürkök, Sümeyra and Özdal, Murat},
title = {Purification and characterization of a novel extracellular, alkaline, thermoactive, and detergent-compatible lipase from Aeromonas caviae LipT51 for application in detergent industry.},
journal = {PROTEIN EXPRESSION AND PURIFICATION},
year = {2021},
volume = {180},
issn = {1046-5928},
doi = {10.1016/j.pep.2021.105819},
url = {https://akademik.atauni.edu.tr/yayinlar/59482-purification-and-characterization-of-a-novel-extracellular-alkaline-thermoactive}
}