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In Vitro Transcriptome Analysis of Cobalt Boride Nanoparticles on Human Pulmonary Alveolar Cells

Mehmet Enes Arslan, Arzu Tatar, Özge Çağlar Yıldırım, İrfan Oğuz Şahin, Ozlem Ozdemir, Erdal Sönmez, Ahmet Hacımüftüoğlu, Metin Acikyildiz, Fatime Geyikoğlu, Adil Mardinoğlu, Hasan Türkez

MATERIALS Cilt 15 · Sayı 23 · 01.12.2022
DOI ile aç

Özet

Nanobiotechnology influences many different areas, including the medical, food, energy, clothing, and cosmetics industries. Considering the wide usage of nanomaterials, it is necessary to investigate the toxicity potentials of specific nanosized molecules. Boron-containing nanoparticles (NPs) are attracting much interest from scientists due to their unique physicochemical properties. However, there is limited information concerning the toxicity of boron-containing NPs, including cobalt boride (Co2B) NPs. Therefore, in this study, Co2B NPs were characterized using X-ray crystallography (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and energy-dispersive X-ray spectroscopy (EDX) techniques. Then, we performed 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH) release, and neutral red (NR) assays for assessing cell viability against Co2B NP exposure on cultured human pulmonary alveolar epithelial cells (HPAEpiC). In addition, whole-genome microarray analysis was carried out to reveal the global gene expression differentiation of HPAEpiC cells after Co2B NP application. The cell viability tests unveiled an IC50 value for Co2B NPs of 310.353 mg/L. The results of our microarray analysis displayed 719 gene expression differentiations (FC >= 2) among the analyzed 40,000 genes. The performed visualization and integrated discovery (DAVID) analysis revealed that there were interactions between various gene pathways and administration of the NPs. Based on gene ontology biological processes analysis, we found that the P53 signaling pathway, cell cycle, and cancer-affecting genes were mostly affected by the Co2B NPs. In conclusion, we suggested that Co2B NPs would be a safe and effective nanomolecule for industrial applications, particularly for medical purposes.

Anahtar kelimeler

IN-VITRO cytotoxicity Pathway analysis Toxicogenomics cobalt boride nanoparticles human pulmonary alveolar epithelial cells

Sürdürülebilir Kalkınma Amaçları

  • SKA 3: Sağlıklı Bireyler

Atıf için künye

Arslan, M. E., Tatar, A., Yıldırım, Ö. Ç., Şahin, İ. O., Ozdemir, O., Sönmez, E., Hacımüftüoğlu, A., Acikyildiz, M., Geyikoğlu, F., Mardinoğlu, A., & Türkez, H. (2022). In Vitro Transcriptome Analysis of Cobalt Boride Nanoparticles on Human Pulmonary Alveolar Cells. MATERIALS, 15(23). https://doi.org/10.3390/ma15238683

BibTeX 
@article{arslan2022-59339,
  author = {Arslan, Mehmet Enes and Tatar, Arzu and Yıldırım, Özge Çağlar and Şahin, İrfan Oğuz and Ozdemir, Ozlem and Sönmez, Erdal and Hacımüftüoğlu, Ahmet and Acikyildiz, Metin and Geyikoğlu, Fatime and Mardinoğlu, Adil and Türkez, Hasan},
  title = {In Vitro Transcriptome Analysis of Cobalt Boride Nanoparticles on Human Pulmonary Alveolar Cells},
  journal = {MATERIALS},
  year = {2022},
  volume = {15},
  number = {23},
  issn = {1996-1944},
  doi = {10.3390/ma15238683},
  url = {https://akademik.atauni.edu.tr/yayinlar/59339-in-vitro-transcriptome-analysis-of-cobalt-boride-nanoparticles-on-human}
}