Total production of (R)-3,5-bistrifluoromethylphenyl ethanol by asymmetric reduction of 3,5-bis(trifluoromethyl)-acetophenone in the submerged culture of Penicillium expansum isolate
Özet
A total of 82 fungal isolates were screened for their ability to reduce 3,5-bis(trifluoromethyl)-acetophenone 1. Penicillium expansum was found to successfully reduce ketone I in the submerged culture. A second screening was performed on the active P. expansum strains identified by the first screening. A number of strains of P. expansum were found to produce (R)-3,5-bistrifluoromethylphenyl ethanol 2 with over 99% enantiomeric excess (ee) from ketone 1. The most productive strain was identified as A expansum EBK-9, and this strain was selected for further experiments. The total production of 2 was carried out by A expansum EBK-9 in a laboratory-scale bioreactor employing optimized conditions as determined by our experiments. P. expansum EBK-9 gave 2 with ee >99% and 76% yield. On a large scale, a total of 3.35 g/L of 2 was produced from I after 56 h. (C) 2009 Elsevier Ltd. All rights reserved.
Atıf için künye
Kurbanoğlu, E. B., Zilbeyaz, K., Taşkın, M., & Kurbanoglu, N. I. (2009). Total production of (R)-3,5-bistrifluoromethylphenyl ethanol by asymmetric reduction of 3,5-bis(trifluoromethyl)-acetophenone in the submerged culture of Penicillium expansum isolate. TETRAHEDRON-ASYMMETRY, 20(23), 2759–2763. https://doi.org/10.1016/j.tetasy.2009.11.006
BibTeX
@article{kurbanoglu2009-59212,
author = {Kurbanoğlu, Esabi Başaran and Zilbeyaz, Kani and Taşkın, Mesut and Kurbanoglu, Namudar I.},
title = {Total production of (R)-3,5-bistrifluoromethylphenyl ethanol by asymmetric reduction of 3,5-bis(trifluoromethyl)-acetophenone in the submerged culture of Penicillium expansum isolate},
journal = {TETRAHEDRON-ASYMMETRY},
year = {2009},
volume = {20},
number = {23},
pages = {2759--2763},
issn = {0957-4166},
doi = {10.1016/j.tetasy.2009.11.006},
url = {https://akademik.atauni.edu.tr/yayinlar/59212-total-production-of-r-35-bistrifluoromethylphenyl-ethanol-by-asymmetric}
}