Identification on Species Level and Density of Aeromonas Bacteria in The Microbiota of Insect at Family of Hydrophilidae and Helophoridae

Insects (hexapods) are the richest group

in terms of species diversity in the World. As a bioindicator in an ecological

spatial, researching microbiota in the digestive system of damaging or useful

hexapods for agricultural production has recently obtained importance for

biological control. It is known that the most important members of hexapod

microbiota are bacteria. Lately, studies based on 16S rRNA gene sequences have

been increasing to identify bacteria with anti-larval effects acquired from

microbiota.<o:p></o:p>

Hexapod collected from different aquatic

environments in and around Erzurum province (Turkey) between May and September

2020. After species identification of the hexapod, it was treated with 70%

alcohol for 5 minutes and surface decontamination was performed to eliminate

possible contaminant microorganisms on the hexapod. The digestive structure was

dissected following aseptic rules in the laboratory. It is purposed to support

the molecular methods in the diagnosis of bacteria with the API (BioMerieux)

system, which is a conventional method. For this reason, the API-20E test,

which is the most suitable API test kit, was applied by taking Gram bacteria

and other biochemical tests into consideration. 16S rDNA amplicons of the

isolates were obtained, sequence analysis was determined, and the derived

sequences were processed and compared with library data

(http://blast.ncbi.nlm.nih.gov/blast.cgi) (Blast analysis). Clinical isolates

were named by evaluating their molecular and conventional data together and

GenBank acceptance numbers were obtained.<o:p></o:p>

Aeromonas

bacteria were the genus with the most samples out of 69 bacteria identified as

a result of this research. Of those of clinical isolates (69), 12 (17%) were

determined. Such as; Aeromonas (4 Aeromonas veronii, 3 Aeromonas allosaccharophila, 4 Aeromonas hydrophila and other Aeromonas rivipollensis). Even though it

is produced in both aerobic and anaerobic environments in isolation processes,

the superiority of aerobic bacteria draws attention. Aeromonas; has been reported to cause infections in humans,

terrestrial and aquatic animals, reptiles and fish. The importance of Aeromonas increases the importance of

extraintestinal infections and sepsis especially in immunocompromised

individuals since they are increasingly included among gastrointestinal

pathogens in recent years.<o:p></o:p>

Our current research is

a preliminary study but more studies and advanced techniques are needed to

define these hexapods microbiota.