Isolation and Identification of Potential Pathogenic Bacteria on Aquatic Insects, An Example Study of Environmental Microbiology In Türkiye, Erzurum Province

Insects, leading the richest group

in species diversity in the world of living organisms, display the largest diversity in feeding, habitat and adaptation to different

living environments. In addition to studies related to the use of insects in

the search for alternative foods in the world in general, the focus has been on

toxicological and pathogenic research about insects. The habitats of aquatic

beetles are the water sources used for both agricultural and drinking water, so

aquatic beetles gain further importance. This study collected aquatic insects from different localities in Erzurum and its surroundings. After

insect species were identified and their digestive tracts were dissected under

aseptic conditions, microbiological analyses were performed. Isolates were

identified by analysing molecular and conventional data together. In addition

to conventional analyses, 16S rRNA gene sequences were amplified with PCR

reaction and DNA sequencing analyses were performed. GenBank accession numbers were obtained after BLAST analyses of the obtained DNA sequences. This study

aimed to identify definite and potential human pathogens in bacteria isolated

from the intestinal microbiota cultures, characterized by conventional and

molecular methods of the aquatic insects.<o:p></o:p>