Identification of Thermophilic Strains from Geothermal Areas in Turkey by using Conventional and Molecular Techniques
Özet
A survey of isolation was practised by obtaining water samples from various thermal facilities in order to find out new types of thermophilic bacteria and to characterize them via phenotypic and genotypic methods. Hence, the test strains were first grouped according to their colonial morphologies and were characterized via conventional (morphological, physiological, biochemical) and molecular [Fatty Acid Methyl Ester profiles (FAME), 16S rRNA sequencing and BOX-PCR] methods. As the result of the research, it was determined that FAMEs method failed in the identification of thermophilic bacteria at the species level whereas BOX-PCR technique was quite successful in exhibiting genetic differences among thermal isolates.
Anahtar kelimeler
Atıf için künye
YANMIS, D., Baltacı, M. Ö., GÜLLÜCE, M., & Adıgüzel, A. (2015). Identification of Thermophilic Strains from Geothermal Areas in Turkey by using Conventional and Molecular Techniques. RESEARCH JOURNAL OF BIOTECHNOLOGY, 10(1), 39–45.
BibTeX
@article{yanmis2015-56937,
author = {YANMIS, Derya and Baltacı, Mustafa Özkan and GÜLLÜCE, Medine and Adıgüzel, Ahmet},
title = {Identification of Thermophilic Strains from Geothermal Areas in Turkey by using Conventional and Molecular Techniques},
journal = {RESEARCH JOURNAL OF BIOTECHNOLOGY},
year = {2015},
volume = {10},
number = {1},
pages = {39--45},
issn = {2278-4535},
url = {https://akademik.atauni.edu.tr/yayinlar/56937-identification-of-thermophilic-strains-from-geothermal-areas-in-turkey-by-using}
}