Palandöken Dağı’ndan İzole Edilen, Antimikrobiyal Ajan Üreten Bakterilerin İzolasyonu Ve Moleküler Karakterizasyonu

Streptomyces is a genus of Gram-positive bacteria that grows in different habitats, and

its shape takes after filamentous fungi. The most effective characteristic of Streptomyces is the

ability to produce secondary metabolites, such as antivirals, antifungals, anti-hypertensives,

antitumorals, and especially antibiotics. In this study, bacteria producing antimicrobial substances

were isolated from soil samples collected from Palandöken Mountain. Test strains were identified

using conventional (morphological, physiological and biochemical tests) and molecular methods

(16S rRNA sequencing). Then, the antagonistic effect of these bacteria against pathogenic

microorganisms was determined by disc diffusion method. As a result of analysis, it was found

that two bacteria (AO1 and AO3) were similar to Streptomyces violaceochromogenes, one (AO2)

to Streptomyces ambofaciens and other (AO4) to Sphingomonas melonis at a rate of 99%.

According to conventional tests, all isolates were catalase positive, three were oxidase negative

(except AO4). In addition, pH, NaCl and temperature values that isolates can growth were

determined. Also, phylogenetic trees of the isolates were performed by the neighbor-joining

method. Finally, the antimicrobial properties of bacteria were investigated. It was determined that

the isolates generally showed high antimicrobial effect against Escherichia coli O157: H7 strain

and the lowest antimicrobial effect was shown against Staphylococcus aureus strain